Sakimura K, Bujo H, Kushiya E, Araki K, Yamazaki M, Yamazaki M, Meguro H, Warashina A, Numa S, Mishina M
Department of Neuropharmacology, School of Medicine, Niigata University, Japan.
FEBS Lett. 1990 Oct 15;272(1-2):73-80. doi: 10.1016/0014-5793(90)80452-o.
The complete amino acid sequences of two mouse glutamate receptor subunits (GluR1 and GluR2) have been deduced by cloning and sequencing the cDNAs. Xenopus oocytes injected with mRNA derived from the GluR1 cDNA exhibit current responses both to kainate and to quisqualate as well as to glutamate, whereas oocytes injected with mRNA derived from the GluR2 cDNA show little response. Injection of oocytes with both the mRNAs produces current responses larger than those induced by the GluR1-specific mRNA and the dose-response relations indicate a positively cooperative interaction between the two subunits. These results suggest that kainate and quisqualate can activate a common glutamate receptor subtype and that glutamate-gated ionic channels are hetero-oligomers of different subunits.
通过对cDNA进行克隆和测序，已推导得出两种小鼠谷氨酸受体亚基（GluR1和GluR2）的完整氨基酸序列。注射了源自GluR1 cDNA的mRNA的非洲爪蟾卵母细胞对海人藻酸、quisqualate以及谷氨酸均表现出电流反应，而注射了源自GluR2 cDNA的mRNA的卵母细胞则几乎没有反应。同时向卵母细胞注射这两种mRNA所产生的电流反应比由GluR1特异性mRNA诱导的反应更大，剂量反应关系表明这两个亚基之间存在正协同相互作用。这些结果表明，海人藻酸和quisqualate可以激活一种共同的谷氨酸受体亚型，并且谷氨酸门控离子通道是不同亚基的异源寡聚体。
