• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

转化生长因子-β中和对铜绿假单胞菌烧伤创面感染小鼠模型生存及细菌清除的影响

Effect of transforming growth factor-beta neutralization on survival and bacterial clearance in a murine model of Pseudomonas aeruginosa burn wound infection.

作者信息

Huang Zhiyu, Pereira Clifford, Toliver-Kinsky Tracy, Murphey Erle D, Varma Tushar K, Lin Cheng Y, Herndon David N, Sherwood Edward R

机构信息

Shriners Hospitals for Children, Galveston, Texas, USA.

出版信息

J Burn Care Res. 2006 Sep-Oct;27(5):682-7. doi: 10.1097/01.BCR.0000238083.95170.2A.

DOI:10.1097/01.BCR.0000238083.95170.2A
PMID:16998401
Abstract

Transforming growth factor-beta (TGF-beta), a cytokine with anti-inflammatory properties, may contribute to postburn immunosuppression. This study was designed to determine whether neutralizing TGF-beta in burned mice could improve resistance to infection. C57BL/6J mice received a 35% TBSA flame burn under isoflurane anesthesia. Four days after injury, mice were treated with TGF-beta antibody or nonspecific IgG. On day 5 after burn injury, mice were inoculated with Pseudomonas aeruginosa at the burn wound site or received intraperitoneal injection with P. aeruginosa. Mice treated with anti-TGF-beta exhibited significantly improved survival compared with mice treated with nonspecific IgG after challenge with P. aeruginosa at the burn wound site or after intraperitoneal injection of P. aeruginosa. In mice with burn wound infections, bacterial counts in burn wounds, blood, and lung were decreased in mice treated with anti-TGF-beta compared with mice treated with control IgG. Bacterial counts in lung and blood after intraperitoneal challenge with P. aeruginosa also were significantly lower in burned mice treated with anti-TGF-beta compared with those treated with nonspecific IgG. Our data suggest that neutralization of TGF-beta at 4 days after burn injury in mice improves local and systemic clearance of P. aeruginosa and enhances survival after P. aeruginosa challenge.

摘要

转化生长因子-β（TGF-β）是一种具有抗炎特性的细胞因子，可能导致烧伤后免疫抑制。本研究旨在确定中和烧伤小鼠体内的TGF-β是否能提高抗感染能力。C57BL/6J小鼠在异氟烷麻醉下接受35%体表面积的火焰烧伤。受伤4天后，小鼠接受TGF-β抗体或非特异性IgG治疗。烧伤后第5天，在烧伤创面接种铜绿假单胞菌或腹腔注射铜绿假单胞菌。在烧伤创面接种铜绿假单胞菌或腹腔注射铜绿假单胞菌后，与接受非特异性IgG治疗的小鼠相比，接受抗TGF-β治疗的小鼠存活率显著提高。在有烧伤创面感染的小鼠中，与接受对照IgG治疗的小鼠相比，接受抗TGF-β治疗的小鼠烧伤创面、血液和肺中的细菌计数减少。与接受非特异性IgG治疗的小鼠相比，接受抗TGF-β治疗的烧伤小鼠在腹腔注射铜绿假单胞菌后的肺和血液中的细菌计数也显著降低。我们的数据表明，在小鼠烧伤后4天中和TGF-β可改善铜绿假单胞菌的局部和全身清除，并提高铜绿假单胞菌攻击后的存活率。

相似文献

1
Effect of transforming growth factor-beta neutralization on survival and bacterial clearance in a murine model of Pseudomonas aeruginosa burn wound infection.转化生长因子-β中和对铜绿假单胞菌烧伤创面感染小鼠模型生存及细菌清除的影响
J Burn Care Res. 2006 Sep-Oct;27(5):682-7. doi: 10.1097/01.BCR.0000238083.95170.2A.
2
Efficacy of antibodies against the N-terminal of Pseudomonas aeruginosa flagellin for treating infections in a murine burn wound model.抗铜绿假单胞菌鞭毛蛋白N端抗体在小鼠烧伤创面模型中治疗感染的疗效。
Plast Reconstr Surg. 2006 Jun;117(7):2284-91. doi: 10.1097/01.prs.0000218768.34429.e0.
3
Protective efficacy of Pseudomonas aeruginosa type-A flagellin in the murine burn wound model of infection.铜绿假单胞菌 A 型鞭毛蛋白对感染性烧伤创面模型的保护作用。
APMIS. 2014 Feb;122(2):115-27. doi: 10.1111/apm.12101. Epub 2013 Jun 12.
4
Effect of thermal injury with Pseudomonas aeruginosa infection on pulmonary and systemic bacterial clearance.铜绿假单胞菌感染所致热损伤对肺部及全身细菌清除的影响。
J Trauma. 1999 Nov;47(5):912-7. doi: 10.1097/00005373-199911000-00016.
5
Blocking TNF-α enhances Pseudomonas aeruginosa-induced mortality in burn mice through induction of IL-1β.阻断 TNF-α 通过诱导 IL-1β 增强烧伤小鼠中铜绿假单胞菌诱导的死亡率。
Cytokine. 2013 Jul;63(1):58-66. doi: 10.1016/j.cyto.2013.04.002. Epub 2013 Apr 25.
6
Passive immunisation against Pseudomonas aeruginosa recombinant flagellin in an experimental model of burn wound sepsis.被动免疫针对铜绿假单胞菌重组菌毛在烧伤创面脓毒症实验模型。
Burns. 2011 Aug;37(5):865-72. doi: 10.1016/j.burns.2010.12.003. Epub 2011 Feb 22.
7
Evaluation of the immune responses following co-administration of PilQ and type b-flagellin from Pseudomonas aeruginosa in the burn mouse model.评价铜绿假单胞菌 PilQ 与 b 型菌毛共给药在烧伤小鼠模型中的免疫应答。
Microb Pathog. 2018 Oct;123:426-432. doi: 10.1016/j.micpath.2018.07.042. Epub 2018 Jul 31.
8
Therapy with anti-flagellin A monoclonal antibody limits Pseudomonas aeruginosa invasiveness in a mouse burn wound sepsis model.抗鞭毛蛋白 A 单克隆抗体治疗可限制铜绿假单胞菌在小鼠烧伤感染性模型中的侵袭性。
Burns. 2009 May;35(3):390-6. doi: 10.1016/j.burns.2008.08.014. Epub 2008 Oct 31.
9
Glucan phosphate treatment attenuates burn-induced inflammation and improves resistance to Pseudomonas aeruginosa burn wound infection.磷酸葡聚糖治疗可减轻烧伤引起的炎症，并提高对铜绿假单胞菌烧伤创面感染的抵抗力。
Shock. 2005 Mar;23(3):224-32.
10
Mouse Model of Burn Wound and Infection: Thermal (Hot Air) Lesion-Induced Immunosuppression.烧伤创面与感染的小鼠模型：热（热风）损伤诱导的免疫抑制。
Curr Protoc Mouse Biol. 2017 Jun 19;7(2):77-87. doi: 10.1002/cpmo.25.

引用本文的文献

1
The Burn Wound Microenvironment.烧伤创面微环境
Adv Wound Care (New Rochelle). 2016 Mar 1;5(3):106-118. doi: 10.1089/wound.2014.0536.
2
The Toll-like receptor 4 agonist monophosphoryl lipid a augments innate host resistance to systemic bacterial infection.Toll 样受体 4 激动剂单磷酰脂质 A 增强固有宿主对全身细菌感染的抵抗力。
Infect Immun. 2011 Sep;79(9):3576-87. doi: 10.1128/IAI.00022-11. Epub 2011 Jun 6.
3
Topical nanoemulsion therapy reduces bacterial wound infection and inflammation after burn injury.局部纳米乳剂疗法可减少烧伤感染后的细菌感染和炎症。
Surgery. 2010 Sep;148(3):499-509. doi: 10.1016/j.surg.2010.01.001. Epub 2010 Mar 2.