Calum Henrik, Høiby Niels, Moser Claus
Department of Clinical Microbiology, Hvidovre Hospital, Copenhagen, Denmark.
Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
Curr Protoc Mouse Biol. 2017 Jun 19;7(2):77-87. doi: 10.1002/cpmo.25.
The immunosuppression induced by thermal injury renders the burned victim susceptible to infection. A mouse model was developed to examine the immunosuppression, which was possible to induce even at a minor thermal insult of 6% total body surface area. After induction of the burn (48 hr) a depression of leukocytes in the peripheral blood was found of the burned mice. This depression was due to a reduction in the polymorphonuclear cells. The burned mice were not able to clear a Pseudomonas aeruginosa wound infection, since the infection spread to the blood as compared to mice only infected with P. aeruginosa subcutaneously. The burn model offers an opportunity to study infections under these conditions. The present model can also be used to examine new antibiotics and immune therapy. Our animal model resembling the clinical situation is useful in developing new treatments of burn wound victims. © 2017 by John Wiley & Sons, Inc.
热损伤所致的免疫抑制使烧伤患者易受感染。开发了一种小鼠模型来研究免疫抑制，即使在6%体表面积的轻微热损伤情况下也有可能诱导出这种免疫抑制。烧伤诱导（48小时）后，发现烧伤小鼠外周血白细胞减少。这种减少是由于多形核细胞数量减少所致。烧伤小鼠无法清除铜绿假单胞菌伤口感染，因为与仅皮下感染铜绿假单胞菌的小鼠相比，感染扩散到了血液中。烧伤模型为研究这些条件下的感染提供了一个机会。目前的模型还可用于检测新抗生素和免疫疗法。我们类似于临床情况的动物模型对于开发烧伤患者的新治疗方法很有用。©2017约翰威立父子公司版权所有
