Kuballa Anna V, Guyatt Kimberley, Dixon Bryony, Thaggard Hazra, Ashton Anthony R, Paterson Brian, Merritt David J, Elizur Abigail
Department of Primary Industries and Fisheries (DPI and F), Animal Science, Bribie Island, Qld 4507, Australia.
Gen Comp Endocrinol. 2007 Jan 1;150(1):48-58. doi: 10.1016/j.ygcen.2006.07.020. Epub 2006 Sep 25.
Farnesoic acid O-methyltransferase (FaMeT) is the enzyme responsible for the conversion of farnesoic acid (FA) to methyl farnesoate (MF) in the final step of MF synthesis. Multiple isoforms of putative FaMeT were isolated from six crustacean species belonging to the families Portunidae, Penaeidae, Scyllaridae and Parastacidae. The portunid crabs Portunus pelagicus and Scylla serrata code for three forms: short, intermediate and long. Two isoforms (short and long) were isolated from the penaeid prawns Penaeus monodon and Fenneropenaeus merguiensis. Two isoforms were also identified in the scyllarid Thenus orientalis and parastacid Cherax quadricarinatus. Putative FaMeT sequences were also amplified from the genomic DNA of P. pelagicus and compared to the putative FaMeT transcripts expressed. Each putative FaMeT cDNA isoform was represented in the genomic DNA, indicative of a multi-gene family. Various tissues from P. pelagicus were individually screened for putative FaMeT expression using PCR and fragment analysis. Each tissue type expressed all three isoforms of putative FaMeT irrespective of sex or moult stage. Protein domain analysis revealed the presence of a deduced casein kinase II phosphorylation site present only in the long isoform of putative FaMeT.
法尼酸O-甲基转移酶（FaMeT）是在甲基法尼酯（MF）合成的最后一步负责将法尼酸（FA）转化为甲基法尼酯（MF）的酶。从属于梭子蟹科、对虾科、蝉虾科和拟螯虾科的六种甲壳类动物中分离出了多种假定的FaMeT同工型。梭子蟹科的远海梭子蟹和锯缘青蟹编码三种形式：短、中、长。从对虾科的斑节对虾和墨吉对虾中分离出两种同工型（短和长）。在蝉虾科的东方扁虾和拟螯虾科的四脊光壳螯虾中也鉴定出了两种同工型。还从远海梭子蟹的基因组DNA中扩增出假定的FaMeT序列，并与所表达的假定FaMeT转录本进行比较。每个假定的FaMeT cDNA同工型都存在于基因组DNA中，表明这是一个多基因家族。使用PCR和片段分析对远海梭子蟹的各种组织分别进行假定FaMeT表达筛选。每种组织类型都表达假定FaMeT的所有三种同工型，与性别或蜕皮阶段无关。蛋白质结构域分析显示，仅在假定FaMeT的长同工型中存在一个推导的酪蛋白激酶II磷酸化位点。
