Schönberg Lena, Grobosch Thomas, Lampe Dagmar, Kloft Charlotte
Berliner Betrieb fuer Zentrale Gesundheitliche Aufgaben (BBGes), Institute of Clinical Toxicology-Clinical Toxicology and Poison Control Center, Oranienburgerstrasse 285, 13437 Berlin, Germany.
J Chromatogr A. 2006 Nov 17;1134(1-2):177-85. doi: 10.1016/j.chroma.2006.09.009. Epub 2006 Sep 26.
A fully automated, qualitative screening HPLC method for the identification of basic compounds in urine has been developed. A 1-ml volume of urine was extracted by on-line extraction and separated on two coupled strong cation-exchange (SCX) columns (2 x LunaSCX, 150 mm x 4.6 mm, 5 microm) under isocratic conditions. The mobile phase consisted of a mixture of potassium dihydrogenphosphate buffer (pH 2.3) and acetonitrile. The use of photodiode-array detection (DAD, lambda = 190-800 nm) gave access to a library of approximately 2600 toxicologically relevant compounds. The validated method is reliable, simple and in addition successfully proven with the analysis of real biological specimen for the routine use.
