Bahar M, Ballard C, Cohen S X, Cowtan K D, Dodson E J, Emsley P, Esnouf R M, Keegan R, Lamzin V, Langer G, Levdikov V, Long F, Meier C, Muller A, Murshudov G N, Perrakis A, Siebold C, Stein N, Turkenburg M G W, Vagin A A, Winn M, Winter G, Wilson K S
The Division of Structural Biology and Oxford Protein Production Facility, The Wellcome Trust Centre for Human Genetics, Oxford, England.
Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1170-83. doi: 10.1107/S0907444906032197. Epub 2006 Sep 19.
The Structural Proteomics In Europe (SPINE) consortium contained a workpackage to address the automated X-ray analysis of macromolecules. The aim of this workpackage was to increase the throughput of three-dimensional structures while maintaining the high quality of conventional analyses. SPINE was able to bring together developers of software with users from the partner laboratories. Here, the results of a workshop organized by the consortium to evaluate software developed in the member laboratories against a set of bacterial targets are described. The major emphasis was on molecular-replacement suites, where automation was most advanced. Data processing and analysis, use of experimental phases and model construction were also addressed, albeit at a lower level.
欧洲结构蛋白质组学（SPINE）联盟有一个工作包，用于处理大分子的自动X射线分析。该工作包的目的是在保持传统分析高质量的同时，提高三维结构的通量。SPINE能够将软件开发者与合作实验室的用户聚集在一起。本文描述了该联盟组织的一次研讨会的结果，该研讨会旨在根据一组细菌靶点评估成员实验室开发的软件。主要重点是分子置换套件，其中自动化程度最高。数据处理与分析、实验相位的使用以及模型构建也得到了讨论，尽管讨论程度较低。
