Zhao Xinping C, Yee Richard W, Norcom Evan, Burgess Heather, Avanesov Andrei S, Barrish Jim P, Malicki Jarema
Department of Ophthalmology and Visual Science and Hermann Eye Center, University of Texas Medical School at Houston, Houston, Texas 77030, USA.
Invest Ophthalmol Vis Sci. 2006 Oct;47(10):4341-8. doi: 10.1167/iovs.05-1611.
To evaluate the zebrafish as a model for the studies of corneal development and disease.
Zebrafish embryos and larvae at various stages of development were used for documenting corneal morphogenesis and differentiation. Corneal samples were collected from embryos, larvae, and adult zebrafish for histologic and electron microscopy analysis. Expression patterns of corneal polypeptides were investigated by immunostaining of sections.
The zebrafish cornea develops rapidly during embryogenesis, so that its three major layers, the epithelium, the stroma, and the endothelium, are well formed by day 3 postfertilization. The subsequent steps of corneal differentiation, such as the thickening of the corneal stroma, proceed relatively slowly. Several polypeptides are highly enriched in the epithelium or the stroma of the larval and adult zebrafish cornea and are excellent markers of corneal differentiation.
Development and differentiation of the zebrafish cornea are easily accessible to analysis. Anatomic and ultrastructural characterization of the zebrafish cornea demonstrates many similarities to the human cornea and provides the basis for the use of the zebrafish model both to analyze the basic genetic mechanisms of corneal development and to study the causes of corneal disease.
评估斑马鱼作为角膜发育和疾病研究模型的价值。
使用处于不同发育阶段的斑马鱼胚胎和幼体记录角膜的形态发生和分化过程。从胚胎、幼体和成体斑马鱼中采集角膜样本进行组织学和电子显微镜分析。通过对切片进行免疫染色研究角膜多肽的表达模式。
斑马鱼角膜在胚胎发育过程中迅速发育，受精后第3天其上皮、基质和内皮这三个主要层就已形成。角膜分化的后续步骤，如角膜基质增厚，进展相对较慢。几种多肽在幼体和成体斑马鱼角膜的上皮或基质中高度富集，是角膜分化的优良标志物。
斑马鱼角膜的发育和分化易于分析。斑马鱼角膜的解剖学和超微结构特征显示出与人类角膜有许多相似之处，为利用斑马鱼模型分析角膜发育的基本遗传机制以及研究角膜疾病的病因提供了基础。
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