Cammarata S, Mancardi G, Tabaton M
Institute of Neurology, University of Genova, Italy.
Neurosci Lett. 1990 Jul 31;115(2-3):351-5. doi: 10.1016/0304-3940(90)90481-n.
In cases of progressive supranuclear palsy (PSP) and Alzheimer's disease (AD) the treatment of tissue sections with formic acid (FA) disclosed a neurofilament epitope in subcortical straight (SF) and paired helical (PHF) filaments. The same treatment produced a two-fold increase of tau-reactive neuropil threads and plaque-related neurites in AD cortical tissue. These findings indicate that epitopes of cytoskeletal proteins are hidden by the beta-pleated conformation of SF and PHF components. FA treatment should be used in immunohistochemical detection of intraneuronal abnormal cytoskeletal filaments.
在进行性核上性麻痹（PSP）和阿尔茨海默病（AD）病例中，用甲酸（FA）处理组织切片后，在皮质下直丝（SF）和双螺旋丝（PHF）中发现了一种神经丝表位。同样的处理使AD皮质组织中tau反应性神经纤维丝和斑块相关神经突增加了两倍。这些发现表明，细胞骨架蛋白的表位被SF和PHF成分的β折叠构象所掩盖。在免疫组织化学检测神经元内异常细胞骨架丝时应使用FA处理。
