Ballini J P, Vigny P, Daniels M
Institut Curie and Université Paris VI, Paris, France.
Biophys Chem. 1983 Jul;18(1):61-5. doi: 10.1016/0301-4622(83)80027-1.
The first lifetime measurements of DNA fluorescence are reported. Natural and synthetic DNA have been excited by 1.76 ns pulses of synchrotron ultraviolet radiation (270 nm) and the time profile of the fluorescence has been measured by synchronous single-photon counting. A post-pulse exponentially decaying emission has been observed with a lifetime of 2.9 +/- 0.4 ns for calf thymus DNA and 3.0 +/- 0.3 ns for poly(dA-T); this is most likely an excimer fluorescence.
首次报道了DNA荧光的寿命测量。天然和合成DNA已被同步加速器紫外线辐射（270nm）的1.76ns脉冲激发，并且通过同步单光子计数测量了荧光的时间分布。观察到脉冲后呈指数衰减的发射，小牛胸腺DNA的寿命为2.9±0.4ns，聚（dA-T）的寿命为3.0±0.3ns；这很可能是准分子荧光。
