de Souza Rosilene C A, Cunha Joice M, Ferreira Sérgio H, Cunha Fernando Q, Lima Hermênio C
Faculdade Evangélica de Medicina--FEPAR, Curitiba, PR 80060-110, Brazil.
Cryobiology. 2006 Dec;53(3):319-29. doi: 10.1016/j.cryobiol.2006.08.003. Epub 2006 Sep 26.
The application of liquid nitrogen to the skin induces inflammation and pain. However, there is little data on the role of inflammatory mediators in the production of these symptoms. We have developed an experimental model to study some aspects of the inflammatory response and its mediators following the application of cold. We have applied liquid nitrogen jets to subcutaneous air pouches in the dorsal skin of rats to study the kinetics of the migration of inflammatory cells; also to the ear for histopathological analysis and on the paws for edema and pain. Inflammatory mediators were identified by pharmacological means. The results showed that the cellular inflammatory response was characterized by persistent cell migration, mainly of granulocytes. Histopathology of the ears confirmed these findings. Histamine and sympathomimetic mediators were mainly responsible for the resultant swelling. However, the hypernociception that resulted involved other mediators including IL-1 and eicosanoids. These data suggest that interference with the release of inflammatory mediators might reduce the side effects of cryosurgery and prevent hyperalgesia and inflammation at the site of application of cold.
将液氮应用于皮肤会引发炎症和疼痛。然而，关于炎症介质在这些症状产生中所起作用的数据很少。我们已经建立了一个实验模型，以研究冷刺激后炎症反应及其介质的某些方面。我们已将液氮喷射到大鼠背部皮肤的皮下气囊中，以研究炎症细胞迁移的动力学；还喷射到耳朵上进行组织病理学分析，并喷射到爪子上以研究水肿和疼痛情况。通过药理学方法鉴定炎症介质。结果表明，细胞炎症反应的特征是细胞持续迁移，主要是粒细胞的迁移。耳朵的组织病理学证实了这些发现。组胺和拟交感神经介质是导致肿胀的主要原因。然而，由此产生的痛觉过敏涉及其他介质，包括白细胞介素 -1 和类花生酸。这些数据表明，干扰炎症介质的释放可能会减少冷冻手术的副作用，并预防冷刺激应用部位的痛觉过敏和炎症。
