Kabeya Hidenori, Yamasaki Akiko, Ikariya Mikako, Negishi Rie, Chomel Bruno B, Maruyama Soichi
Laboratory of Veterinary Public Health, Department of Veterinary Medicine, College of Bioresource Science, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan.
Vet Microbiol. 2007 Jan 31;119(2-4):290-6. doi: 10.1016/j.vetmic.2006.08.010. Epub 2006 Aug 14.
This study was conducted to analyze cytokine production mechanisms in mice after Bartonella henselae stimulation. BALB/c mice were inoculated intraperitoneally with 3 x 10(6) colony forming units of B. henselae (Houston-1 strain) twice at 10-day interval. Spleen cells were harvested from the mice and stimulated with the organisms. Following the stimulation, interferon-gamma (IFN-gamma) and interleukin-4 (IL-4), IL-10, IL-12 and tumor necrosis factor-alpha (TNF-alpha) were measured in the culture supernatants of the spleen cells by ELISA. The spleen cells specifically secreted IFN-gamma, but not IL-4, indicating that T helper 1 (Th1) cells were activated following B. henselae stimulation. In addition, IL-10 and TNF-alpha productions were also detected in the culture supernatants of spleen cells. Neutralization of IL-10 in the culture supernatants significantly enhanced the production of IFN-gamma from the spleen cells stimulated with B. henselae. These results indicate that B. henselae predominantly stimulated Th1 cells and resulted in secreting IFN-gamma, however the production was partially inhibited by IL-10, which was produced simultaneously.
本研究旨在分析亨氏巴尔通体刺激后小鼠体内细胞因子的产生机制。将3×10(6)个菌落形成单位的亨氏巴尔通体（休斯顿-1株）以10天的间隔腹腔接种BALB/c小鼠两次。从小鼠体内采集脾细胞并用该菌进行刺激。刺激后，通过酶联免疫吸附测定法（ELISA）检测脾细胞培养上清液中的干扰素-γ（IFN-γ）、白细胞介素-4（IL-4）、IL-10、IL-12和肿瘤坏死因子-α（TNF-α）。脾细胞特异性分泌IFN-γ，但不分泌IL-4，这表明亨氏巴尔通体刺激后辅助性T细胞1（Th1）被激活。此外，在脾细胞培养上清液中也检测到了IL-10和TNF-α的产生。在培养上清液中中和IL-10可显著增强经亨氏巴尔通体刺激的脾细胞产生IFN-γ。这些结果表明，亨氏巴尔通体主要刺激Th1细胞并导致IFN-γ的分泌，然而该分泌受到同时产生的IL-10的部分抑制。
