Sliwa-Tomczok W, Tomczok J
Department of Electron Microscopy, Silesian Academy of Medicine, Katowice, Poland.
Z Mikrosk Anat Forsch. 1990;104(3):458-64.
The distribution of lead ions in rat peripheral neutrophils was investigated using Timm sulphide silver method. In the neutrophils of adult male rats the Pb-precipitates were electron microscopically localized at 1.5, 3.0 and 6.0 hours after a single intraperitoneal injection of lead at dose 150 mgPb kg b. w. After increased lead ions input to cell matrix a formation of cytoplasmic metal deposits as well as an aggregation of Pb-complexes within deep invaginations of nuclear membrane was detectable. Furthermore, the neutrophils were prepared to examination by scanning electron microscope according to Domagała et al. (1979). In neither case there was a clear difference of neutrophil surface morphology between exposed and control populations. The same preparations were then used to X-ray probe microanalysis, and correlation between the presence of Timm reaction product and lead indication was obtained.
采用Timm硫化银法研究了大鼠外周血中性粒细胞中铅离子的分布。成年雄性大鼠单次腹腔注射剂量为150mgPb/kg体重的铅后，在1.5、3.0和6.0小时用电镜观察到中性粒细胞中的铅沉淀物。铅离子进入细胞基质增加后，可检测到细胞质金属沉积物的形成以及核膜深凹陷内铅复合物的聚集。此外，根据Domagała等人（1979年）的方法制备中性粒细胞用于扫描电子显微镜检查。在暴露组和对照组中，中性粒细胞表面形态均无明显差异。然后将相同的标本用于X射线探针微分析，并获得了Timm反应产物的存在与铅指示之间的相关性。
