枯草芽孢杆菌中11个假定必需基因的功能分析。

Functional analysis of 11 putative essential genes in Bacillus subtilis.

作者信息

Hunt Alison, Rawlins Joy P, Thomaides Helena B, Errington Jeff

机构信息

Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK.

Prolysis Ltd, Oxford University Begbroke Science Park, Sandy Lane, Yarnton, Oxfordshire OX5 1PF, UK.

出版信息

Microbiology (Reading). 2006 Oct;152(Pt 10):2895-2907. doi: 10.1099/mic.0.29152-0.

DOI:10.1099/mic.0.29152-0

PMID:17005971

Abstract

Systematic inactivation of Bacillus subtilis genes has previously revealed that 271 are indispensable for growth. In the present study, 11 of these (yacA, ydiB, ydiC, ykqC, ylaN, yloQ, ymdA, yneS, yqeI, yqjK and ywlC) were identified as genes encoding proteins of unknown function. By analysing the effects of protein depletion, and examining the subcellular localization of these proteins, a start has been made in elucidating their functions. It was found that four of these genes (ydiB, yloQ, yqeI and ywlC) were not required for B. subtilis viability. Analysis of the localization of YkqC suggests that it co-localizes with ribosomes, and it is proposed that it is involved in processing either rRNA or specific mRNAs when they are associated with the ribosome. The results suggest that other novel essential proteins may be involved in lipid synthesis and control of cell wall synthesis.

摘要

先前对枯草芽孢杆菌基因进行的系统性失活研究表明，有271个基因对其生长不可或缺。在本研究中，其中11个基因（yacA、ydiB、ydiC、ykqC、ylaN、yloQ、ymdA、yneS、yqeI、yqjK和ywlC）被鉴定为编码功能未知蛋白质的基因。通过分析蛋白质缺失的影响，并检查这些蛋白质的亚细胞定位，已开始阐明它们的功能。结果发现，其中4个基因（ydiB、yloQ、yqeI和ywlC）并非枯草芽孢杆菌生存所必需。对YkqC定位的分析表明，它与核糖体共定位，并且有人提出，当rRNA或特定mRNA与核糖体结合时，它参与对它们的加工。结果表明，其他新的必需蛋白质可能参与脂质合成和细胞壁合成的控制。