使用1-苯胺基-8-萘磺酸盐作为胃小泡转运的探针。

Use of 1-anilino-8-naphthalene-sulfonate as a probe of gastric vesicle transport.

作者信息

Lewin M, Saccomani G, Schackmann R, Sachs G

出版信息

J Membr Biol. 1977 Apr 22;32(3-4):301-18. doi: 10.1007/BF01905224.

DOI:10.1007/BF01905224

Abstract

The interaction of 1-anilino-8-naphthalene-sulfonate (ANS) with vesicles derived from hog fundic mucosa was studied in the presence of valinomycin and with the addition of ATP. Evidence was found for two classes of sites, those rapidly accessible to ANS with a KD of 7.5 micronM and those slowly accessible, but rapidly accessed in the presence of valinomycin with a KD of 2.5 micronM. ATP transiently increases the quantum yield of the latter ANS binding sites only in the presence of valinomycin, but does not alter the number of KD of those sites. The time course of this increase correlates with H+ uptake and Rb+ extrusion by those vesicles and H+ carries such as tetrachlorsalicylanilide or nigericin abolish the ATP response. With ATP addition in the presence of SC14N and valinomycin there is transient uptake of SCN-. It is concluded that ANS is acting as a probe of a structural change dependent on a potential and H+ gradient.

摘要

在缬氨霉素存在的情况下，并添加ATP，研究了1-苯胺基-8-萘磺酸盐（ANS）与源自猪胃底黏膜的囊泡之间的相互作用。发现存在两类位点，一类是ANS可快速接近的，其解离常数（KD）为7.5微摩尔，另一类是缓慢可接近的，但在缬氨霉素存在下可快速接近，其KD为2.5微摩尔。仅在缬氨霉素存在时，ATP会短暂增加后一类ANS结合位点的量子产率，但不会改变这些位点的KD值或数量。这种增加的时间进程与这些囊泡对H⁺的摄取和Rb⁺的外排相关，并且H⁺载体如四氯水杨酰苯胺或尼日利亚菌素会消除ATP反应。在SC14N和缬氨霉素存在下添加ATP时，会有SCN⁻的短暂摄取。得出的结论是，ANS作为一种依赖于电位和H⁺梯度的结构变化的探针。