Ohyashiki T, Kodera M, Mohri T
J Biochem. 1984 Sep;96(3):665-70. doi: 10.1093/oxfordjournals.jbchem.a134883.
It has been found in the brush border membrane vesicles from porcine small intestine that valinomycin causes vesicle aggregation and that Ca2+-induced aggregation of the vesicles is saturably stimulated in the presence of valinomycin. The apparent half-maximal concentration of valinomycin required to enhance the Ca2+ effect on the membrane aggregation is approximately 40 microM. Results of a fluorometric study using 1-anilino-8-naphthalene sulfonate (ANS) showed that the addition of valinomycin to the membranes induces slight decreases in the ANS-binding affinities for the membranes in the presence and absence of Ca2+. On the other hand, measurements of the incorporation rate of 1,6-diphenyl-1,3,5-hexatriene (DPH) into the vesicles and of anisotropies of DPH-labeled membranes suggested that the lipid organization of the membranes was not altered upon the addition of valinomycin. From these results, it was suggested that the valinomycin effect on the membrane aggregation is mainly related to the nature of the membrane surface charge.
已发现，在猪小肠的刷状缘膜囊泡中，缬氨霉素会导致囊泡聚集，并且在缬氨霉素存在的情况下，Ca2 +诱导的囊泡聚集受到饱和刺激。增强Ca2 +对膜聚集作用所需的缬氨霉素的表观半数最大浓度约为40 microM。使用1-苯胺基-8-萘磺酸盐（ANS）的荧光研究结果表明，在存在和不存在Ca2 +的情况下，向膜中添加缬氨霉素会导致ANS与膜的结合亲和力略有降低。另一方面，对1,6-二苯基-1,3,5-己三烯（DPH）掺入囊泡的速率以及DPH标记膜的各向异性的测量表明，添加缬氨霉素后膜的脂质组织未发生改变。从这些结果表明，缬氨霉素对膜聚集的影响主要与膜表面电荷的性质有关。
