促肾上腺皮质激素释放激素对女性股部脂肪组织代谢的体内效应。

In vivo effects of corticotropin-releasing hormone on femoral adipose tissue metabolism in women.

作者信息

Wellhöner P, Welzel M, Rolle D, Dodt C

机构信息

Department of Internal Medicine I, Universitaetsklinikum Schleswig Holstein-Campus Lübeck, Lübeck, Germany.

出版信息

Int J Obes (Lond). 2007 Apr;31(4):718-22. doi: 10.1038/sj.ijo.0803463. Epub 2006 Sep 26.

DOI:10.1038/sj.ijo.0803463

PMID:17006439

Abstract

OBJECTIVE

To investigate whether i.v. injected corticotropin-releasing hormone (CRH) (1 microg/kg) has a direct effect on adipose tissue metabolism in humans.

DESIGN

Double-blinded, placebo-controlled, crossover study.

SUBJECTS

Twelve healthy normal weight female volunteers (age 20-37 years, body mass index: 22.75+/-1.33 kg/m(2))

MEASUREMENTS

Assessment of local generation of glycerol, and glucose in adipose tissue by microdialysis. Measurement of adipose tissue and skin blood flow by laser Doppler flowmetry.

RESULTS

Injection of CRH acutely increases interstitial concentrations of glycerol (19.0+/-5.4%, P<0.05) and glucose (13.5+/-5.8%, P<0.05) reaching peak levels after 15 min. Plasma glycerol increases in parallel (Delta=16.7+/-5.9% after 15 min (P<0.05)), whereas plasma glucose remains unaffected. Changes in tissue blood flow do not explain interstitial metabolite alterations. Initial CRH effects on adipose tissue metabolism are short lasting and disappear after 15 min.

CONCLUSIONS

The importance of CRH on human energy metabolism is underlined by the present in vivo study demonstrating peptidergic effects on lipolysis and glucose homeostasis in human subcutaneous adipose tissue.

摘要

目的

研究静脉注射促肾上腺皮质激素释放激素（CRH）（1微克/千克）是否对人体脂肪组织代谢有直接影响。

设计

双盲、安慰剂对照、交叉研究。

受试者

12名健康的正常体重女性志愿者（年龄20 - 37岁，体重指数：22.75±1.33千克/平方米）

测量指标

通过微透析评估脂肪组织中甘油和葡萄糖的局部生成。通过激光多普勒血流仪测量脂肪组织和皮肤血流量。

结果

注射CRH后，甘油（19.0±5.4%，P<0.05）和葡萄糖（13.5±5.8%，P<0.05）的间质浓度急性升高，15分钟后达到峰值水平。血浆甘油平行升高（15分钟后变化量=16.7±5.9%（P<0.05）），而血浆葡萄糖不受影响。组织血流量的变化无法解释间质代谢物的改变。CRH对脂肪组织代谢的初始作用持续时间较短，15分钟后消失。