Ohno J, Fukuyama K, Epstein W L
Department of Oral Pathology, School of Dentistry, University of Meikai, Saitama, Japan.
Anat Rec. 1990 Sep;228(1):1-6. doi: 10.1002/ar.1092280102.
Changes in the expression of glycoconjugates in cells of the inner root sheath (IRS) and outer root sheath (ORS) of human anagen hair follicles were investigated by lectin histochemistry. Concanavalin A (Con A) and Ricinus communis (RCA-I) stained hair follicle cells regardless of their differentiation stages. In IRS, Ulex europeaus-I (UEA-I) bound to the surface of the cells as soon as they were morphologically defined, and Glycine max (SBA) stained as their differentiation progressed. Innermost (IM) cells of ORS layers were reactive with UEA-I at the stage where Henle's cells were keratinized, while the reactivity of UEA-I was lost at the site of the completion of IRS keratinization where SBA reaction was detected. Staining of both UEA-I and SBA was prominent in other ORS cells at the levels where SBA binding in IM cells became strong. The staining intensity increased up to the position of the follicular isthmus. In addition, a sugar residue recognized by Dolichos biflorus (DBA) was detected in differentiated cells of ORS. In contrast, the DBA reaction was not found at all in cells of IRS, infundibulum, and epidermis. These findings identified a complexity of carbohydrate metabolism in the cells of different layers at various stages of keratinization. IM cells differentiate independently from other ORS cells but seem responsive to the degree of IRS keratinization. All ORS cells possess a unique sugar moiety not found in other keratinocytes either in the hair or epidermis.
通过凝集素组织化学研究了人生长期毛囊内根鞘（IRS）和外根鞘（ORS）细胞中糖缀合物表达的变化。伴刀豆球蛋白A（Con A）和蓖麻凝集素（RCA-I）对毛囊细胞进行染色，无论其分化阶段如何。在IRS中，欧洲荆豆凝集素-I（UEA-I）一旦细胞在形态上确定，就会结合到细胞表面，随着其分化进展大豆凝集素（SBA）进行染色。ORS层的最内层（IM）细胞在亨勒细胞角质化阶段与UEA-I发生反应，而在检测到SBA反应的IRS角质化完成部位，UEA-I的反应性消失。在IM细胞中SBA结合变强的水平上，UEA-I和SBA在其他ORS细胞中的染色都很明显。染色强度一直增加到毛囊峡部的位置。此外，在ORS的分化细胞中检测到了双花扁豆凝集素（DBA）识别的糖残基。相反，在IRS、漏斗部和表皮的细胞中完全没有发现DBA反应。这些发现确定了在角质化不同阶段不同层细胞中碳水化合物代谢的复杂性。IM细胞与其他ORS细胞独立分化，但似乎对IRS角质化程度有反应。所有ORS细胞都具有一种在毛发或表皮的其他角质形成细胞中未发现的独特糖部分。
