Ito T, Newkirk C, Strum J M, McDowell E M
Department of Anatomy, University of Maryland School of Medicine, Baltimore 21201.
Anat Rec. 1990 Oct;228(2):151-62. doi: 10.1002/ar.1092280207.
Lectin binding was studied in the developing airways of Syrian golden hamsters on gestational days 11-16 (day 16 is the day of birth). The trachea and lungs were fixed in 4% formaldehyde-1% glutaraldehyde, 6% mercuric chloride-1% sodium acetate-0.1% glutaraldehyde, and 95% ethanol; embedded in paraffin; and stained with eight lectin-horseradish peroxidase conjugates: Triticum vulgare (WGA), Dolichos biflorus (DBA), Helix pomatia (HPA), Maclura pomifera (MPA), Griffonia simplicifolia I-B4 (GSA I-B4), Arachis hypogaea (PNA), Ulex europeus I (UEA I), and Limulus polyphemus (LPA). Each lectin yielded a characteristic staining pattern, which modulated throughout development. In general, changes in staining characteristics of the tracheal epithelium preceded similar changes in the lobar bronchus, bronchiole, and alveolus. In the case of UEA I, MPA, WGA, and HPA, staining increased with time uniformly over the luminal surface of all epithelial cells. However, in the case of PNA, GSA I-B4, and LPA, after the differentiation of ciliated and secretory cells, the apical surfaces of the ciliated cells stained more intensely than the apical surfaces of the secretory cells. Neuraminidase pretreatment enhanced PNA and GSA I-B4 staining in both cell types. In the case of PNA, these light microscopic observations were confirmed by ultrastructural study. Unlike the other lectins, the pattern of staining with DBA was unusual. Staining was moderate at first, then decreased (days 13 and 14), then increased at all airway levels. This study shows that different glycoconjugates modulate in airway epithelial cells throughout fetal development.
在妊娠第11 - 16天（第16天为出生日）的叙利亚金黄地鼠发育中的气道中研究了凝集素结合情况。气管和肺分别用4%甲醛 - 1%戊二醛、6%氯化汞 - 1%醋酸钠 - 0.1%戊二醛以及95%乙醇固定；石蜡包埋；并用八种凝集素 - 辣根过氧化物酶偶联物染色：普通小麦（WGA）、双花扁豆（DBA）、苹果螺（HPA）、桑科柘属植物（MPA）、西非单叶豆I - B4（GSA I - B4）、落花生（PNA）、欧洲荆豆I（UEA I）和鲎（LPA）。每种凝集素都产生了一种特征性的染色模式，且在整个发育过程中发生变化。一般来说，气管上皮染色特征的变化先于叶支气管、细支气管和肺泡的类似变化。就UEA I、MPA、WGA和HPA而言，染色在所有上皮细胞的腔表面随时间均匀增加。然而，就PNA、GSA I - B4和LPA而言，在纤毛细胞和分泌细胞分化后，纤毛细胞的顶端表面比分泌细胞的顶端表面染色更深。神经氨酸酶预处理增强了两种细胞类型中PNA和GSA I - B4的染色。就PNA而言，这些光学显微镜观察结果通过超微结构研究得到证实。与其他凝集素不同，DBA的染色模式不寻常。起初染色适中，然后减少（第13天和第14天），然后在所有气道水平上增加。这项研究表明，在胎儿发育过程中，不同的糖缀合物在气道上皮细胞中发挥调节作用。
