Kawai T, Greenberg S D, Titus J L
Department of Pathology, Baylor College of Medicine, Houston, Texas.
Mod Pathol. 1988 Nov;1(6):485-92.
In order to evaluate the staining pattern of glycoconjugate profiles in adenocarcinomas of the lung, pulmonary adenocarcinomas were classified according to their: (a) degree of differentiation; (b) cellular subtyping and mucus secretion; and (c) immunohistochemical characteristics. Studies were performed on 42 pulmonary adenocarcinomas using eight lectins. Formalin-fixed, paraffin-embedded tissues were stained with avidin-biotin peroxidase complex methods. Four lectins [wheat germ (WGA), succinylated WGA (SucWGA), peanut (PNA) with neuraminidase (N) treatment, and Ricinus communis (RCA-I)] showed strong positive staining reactions in well-differentiated adenocarcinomas. Bronchial surface epithelial type, one of the subtypes among 26 cases of well- and moderately differentiated adenocarcinomas, displayed strong positive staining for WGA, SucWGA, PNA N(+), RCA-I, and Bandeirea simplicifolia (BSA-I). Goblet cell types stained positive for all lectins except Dolichos biflorus (DBA). Bronchial gland cell types also showed a strongly positive stain for WGA, SucWGA, soybean (SBA), PNA N(+), RCA-I, and Ulex europaeus (UEA-I). The lectin positive staining reaction was related to the degree of mucus secretion within the tumor cells. These results revealed that the glycoconjugate profile of pulmonary adenocarcinomas was basically sialic acid, together with N-acetyl-glucosamine and beta-D-galactose. The observation that UEA-I showed a strong staining reaction in mucus-producing adenocarcinomas, such as goblet cell and bronchial gland cell types, indicates that localization of alpha-L-fucose may be a specific carbohydrate from non-mucus-producing pulmonary adenocarcinomas.
为了评估肺腺癌中糖缀合物谱的染色模式，肺腺癌根据以下因素进行分类：(a)分化程度；(b)细胞亚型和黏液分泌；以及(c)免疫组化特征。使用八种凝集素对42例肺腺癌进行了研究。用抗生物素蛋白-生物素过氧化物酶复合物方法对福尔马林固定、石蜡包埋的组织进行染色。四种凝集素[麦胚凝集素（WGA）、琥珀酰化WGA（SucWGA）、经神经氨酸酶（N）处理的花生凝集素（PNA）和蓖麻凝集素（RCA-I）]在高分化腺癌中显示出强阳性染色反应。支气管表面上皮型是26例高分化和中分化腺癌中的一种亚型，对WGA、SucWGA、PNA N(+)、RCA-I和单叶豆凝集素（BSA-I）呈强阳性染色。杯状细胞型除了对双花扁豆凝集素（DBA）外，对所有凝集素均呈阳性染色。支气管腺细胞型对WGA、SucWGA、大豆凝集素（SBA）、PNA N(+)、RCA-I和欧洲荆豆凝集素（UEA-I）也呈强阳性染色。凝集素阳性染色反应与肿瘤细胞内的黏液分泌程度有关。这些结果表明，肺腺癌的糖缀合物谱主要是唾液酸，以及N-乙酰葡糖胺和β-D-半乳糖。UEA-I在黏液产生性腺癌（如杯状细胞和支气管腺细胞型）中显示出强染色反应，这一观察结果表明，α-L-岩藻糖的定位可能是来自非黏液产生性肺腺癌的一种特异性碳水化合物。
