苎麻根提取物对HepG2 2.2.15细胞中乙型肝炎病毒产生的抑制作用。
Inhibition of hepatitis B virus production by Boehmeria nivea root extract in HepG2 2.2.15 cells.
作者信息
Huang Kai-Ling, Lai Yiu-Kay, Lin Chih-Chien, Chang Jia-Ming
机构信息
Division of Research and Development, Development Center for Biotechnology, Xizhi City, Taipei County, Taiwan 221, China.
出版信息
World J Gastroenterol. 2006 Sep 21;12(35):5721-5. doi: 10.3748/wjg.v12.i35.5721.
AIM
To explore the anti-hepatitis B virus (HBV) effects of Boehmeria nivea (B. nivea) root extract (BNE) by using the HepG2 2.2.15 cell model system.
METHODS
Hepatitis B surface antigen (HBsAg), hepatitis B virus e antigen (HBeAg), and HBV DNA were measured by using ELISA and real-time PCR, respectively. Viral DNA replication and RNA expression were determined by using Southern and Northern blot, respectively.
RESULTS
In HepG2 2.2.15 cells, HBeAg (60%, P < 0.01) and particle-associated HBV DNA (> 99%, P < 0.01) secretion into supernatant were significantly inhibited by BNE at a dose of 100 mg/L, whereas the HBsAg was not inhibited. With different doses of BNE, the reduced HBeAg was correlated with the inhibition of HBV DNA. The anti-HBV effect of BNE was not caused by its cytotoxicity to cells or inhibition of viral DNA replication and RNA expression.
CONCLUSION
BNE could effectively reduce the HBV production and its anti-HBV machinery might differ from the nucleoside analogues.
目的
利用HepG2 2.2.15细胞模型系统探讨苎麻根提取物（BNE）的抗乙型肝炎病毒（HBV）作用。
方法
分别采用酶联免疫吸附测定法（ELISA）和实时荧光定量聚合酶链反应（real-time PCR）检测乙型肝炎表面抗原（HBsAg）、乙型肝炎e抗原（HBeAg）及HBV DNA。分别采用Southern印迹法和Northern印迹法检测病毒DNA复制和RNA表达。
结果
在HepG2 2.2.15细胞中，100 mg/L剂量的BNE可显著抑制HBeAg（60%，P<0.01）和颗粒相关HBV DNA（>99%，P<0.01）分泌到上清液中，而对HBsAg无抑制作用。不同剂量的BNE作用下，HBeAg的降低与HBV DNA的抑制相关。BNE的抗HBV作用并非由其对细胞的细胞毒性或对病毒DNA复制及RNA表达的抑制所致。
结论
BNE可有效降低HBV的产生，其抗HBV机制可能与核苷类似物不同。
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