Monji N, Cole C A, Tam M, Goldstein L, Nowinski R C
Genetic Systems Corporation, Seattle, WA 98121.
Biochem Biophys Res Commun. 1990 Oct 30;172(2):652-60. doi: 10.1016/0006-291x(90)90724-2.
We have developed a novel method to immobilize antibodies onto a cellulose acetate membrane using a conjugate of an N-isopropylacrylamide polymer covalently bound to the antibody. When compared with the unconjugated antibody, over 30-fold increase in retention of the antibody on the membrane was observed when it was conjugated to poly (N-isopropylacrylamide). Studies of the polymer-membrane interaction suggest a combination of hydrophobic and ionic forces, especially the former, is responsible for the high retention. We applied this novel immobilization technology in the development of a membrane-based immunoassay.
我们开发了一种新方法，利用与抗体共价结合的N-异丙基丙烯酰胺聚合物共轭物将抗体固定在醋酸纤维素膜上。与未共轭的抗体相比，当抗体与聚（N-异丙基丙烯酰胺）共轭时，在膜上的保留率增加了30倍以上。聚合物-膜相互作用的研究表明，疏水和离子力的结合，尤其是前者，是高保留率的原因。我们将这种新型固定技术应用于基于膜的免疫分析的开发。
