使用稳态化学计量通量平衡方法对活细胞代谢进行数学建模。

Mathematical modeling of living cell metabolism using the method of steady-state stoichiometric flux balance.

作者信息

Drozdov-Tikhomirov L N, Scurida G I, Davidov A V, Alexandrov A A, Zvyagilskaya R A

机构信息

Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov sq 2, Moscow 123182, Russia.

出版信息

J Bioinform Comput Biol. 2006 Aug;4(4):865-85. doi: 10.1142/s0219720006002247.

DOI:10.1142/s0219720006002247

PMID:17007072

Abstract

This approach uses a set of algebraic linear equations for reaction rates (the method of steady-state stoichiometric flux balance) to model the purposeful metabolism of the living self-reproducing biochemical system (i.e. cell), which persists in steady-state growth. Linear programming (SIMPLEX method) is used to derive the solution for the model equations set (determining reaction rates which provide flux balance at given conditions). Here, we demonstrate the approach through the mathematical modeling of steady-state metabolism in Saccharomyces cerevisiae mitochondria.

摘要

这种方法使用一组关于反应速率的代数线性方程（稳态化学计量通量平衡法）来模拟活的自我复制生化系统（即细胞）的有目的代谢，该系统在稳态生长中持续存在。线性规划（单纯形法）用于推导模型方程组的解（确定在给定条件下提供通量平衡的反应速率）。在此，我们通过对酿酒酵母线粒体稳态代谢的数学建模来展示这种方法。