[透明质酸壳聚糖基微乳对小鼠血脑屏障通透性的影响]
[Effect of hyaluronic acid chitosan-based microemulsion on the permeability of blood brain barrier in mice].
作者信息
Yao Jing, Zhou Jian-ping, Ping Qi-neng, Lu Yun, Yu Li
机构信息
Department of Pharmaceutics, China Pharmaceutical University, Nanjing 210009, China.
出版信息
Yao Xue Xue Bao. 2006 Jul;41(7):615-8.
AIM
To study the effect of hyaluronic acid chitosan-based microemulsion (HAC-ME) on the permeability of blood brain barrier( BBB) by using Evans blue (EB) as the indicator.
METHODS
A formamide extraction-ultraviolet spectrophotometry method was employed to determine the concentrations of EB in each of the tissues. The in vivo distribution of HAC-ME groups containing EB in mice and the fluorescence intensity and diffusion domain of brain slices were all studied.
RESULTS
Contrasting to the common microemulsion (ME), HAC-ME at the lower concentration of HAC (<5 mg x mL(-1)) could further improve the transporting of EB across the BBB while EB concentration in other tissues decreased, and Tmax was delayed about 30 min.
CONCLUSION
HAC-ME could facilitate the transporting of EB across the BBB and it was concentration dependent. While the brain targeting absorptive capability of HAC-ME was enhanced.
目的
以伊文思蓝（EB）为指示剂，研究透明质酸壳聚糖基微乳（HAC-ME）对血脑屏障（BBB）通透性的影响。
方法
采用甲酰胺萃取-紫外分光光度法测定各组织中EB的浓度。研究了含EB的HAC-ME组在小鼠体内的分布以及脑切片的荧光强度和扩散区域。
结果
与普通微乳（ME）相比，较低浓度HAC（<5 mg·mL⁻¹）的HAC-ME能进一步促进EB跨BBB转运，同时其他组织中EB浓度降低，达峰时间（Tmax）延迟约30分钟。
结论
HAC-ME能促进EB跨BBB转运，且具有浓度依赖性。同时HAC-ME的脑靶向吸收能力增强。
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