Ryder M I, Weinreb R N
Department of Oral Biology, University of California, San Francisco.
Invest Ophthalmol Vis Sci. 1990 Nov;31(11):2230-4.
The authors examined immunofluorescently the specific cytokeratin staining patterns of corneal, limbal, and conjunctival epithelium with PKK-1, 8.12, 4.62, and 8.60 monoclonal anticytokeratin antibodies. Observations were made on unfixed frozen postmortem human tissue. The PKK-1 antibody stain was observed in all layers of corneal epithelium but only in suprabasal layers of limbal and conjunctival epithelium. By contrast, the 8.12 antibody stain was observed only in the superficial layer of corneal epithelium but through all layers of limbal and conjunctival epithelium. The 4.60 antibody stain was seen in focal areas of corneal and limbal epithelium and through all layers of conjunctival epithelium. The 8.60 antibody stain was not present in the three epithelia. These immunofluorescence studies showed unique cytokeratin patterns among layers in corneal, limbal, and conjunctival epithelium.
作者使用PKK-1、8.12、4.62和8.60单克隆抗细胞角蛋白抗体，通过免疫荧光法检测了角膜、角膜缘和结膜上皮细胞角蛋白的特异性染色模式。观察对象为未经固定的死后人体冷冻组织。PKK-1抗体染色在角膜上皮各层均可见，但仅在角膜缘和结膜上皮的基底上层可见。相比之下，8.12抗体染色仅在角膜上皮表层可见，但在角膜缘和结膜上皮各层均可见。4.60抗体染色见于角膜和角膜缘上皮的局灶区域以及结膜上皮各层。8.60抗体染色在这三种上皮中均未出现。这些免疫荧光研究显示了角膜、角膜缘和结膜上皮各层中独特的细胞角蛋白模式。
