基于DNA序列直接从一名血培养阴性的心内膜炎患者心脏瓣膜组织中检测B族链球菌。

DNA sequence-based detection of group B Streptococcus directly from heart valve tissue in a patient with culture-negative endocarditis.

作者信息

Guggisberg Kelly, Parker Robin, Megran David, Zhang Kunyan, Lester Wanda, Elsayed Sameer

机构信息

Department of Pathology and Laboratory Medicine, Calgary, Alberta, Canada.

出版信息

Scand J Infect Dis. 2006;38(10):931-4. doi: 10.1080/00365540600561793.

DOI:10.1080/00365540600561793

PMID:17008243

Abstract

A human case of culture-negative bacterial endocarditis is presented where conventional methods failed to determine a microbial aetiology. DNA sequencing performed directly on autopsy heart valve tissue revealed Streptococcus agalactiae (group B streptococcus). To our knowledge, this is the first report of DNA sequence-based detection of this organism directly from a heart valve.

摘要

本文报告一例血培养阴性的细菌性心内膜炎病例，传统方法未能确定其微生物病因。直接对尸检得到的心脏瓣膜组织进行DNA测序，结果显示为无乳链球菌（B组链球菌）。据我们所知，这是首次直接从心脏瓣膜中通过DNA序列检测出该病原体的报告。