tRNA核苷酸转移酶催化CMP和AMP掺入RNA噬菌体基因组片段。

tRNA nucleotidyltransferase-catalyzed incorporation of CMP and AMP into RNA-bacteriophage genome fragments.

作者信息

Prochiantz A, Bénicourt C, Carré D, Haenni A L

出版信息

Eur J Biochem. 1975 Mar 3;52(1):17-23. doi: 10.1111/j.1432-1033.1975.tb03968.x.

DOI:10.1111/j.1432-1033.1975.tb03968.x

Abstract

Fragments of bacteriophage RNAs R17, MS2 and Qbeta obtained by incubation with commercial snake venom phosphodiesterase become substrates of the Escherichia coli tRNA nucleotidyltransferase. The transferase adds back CMP and AMP in conditions in which it remains highly specific of CCA-deprived tRNAs. The results suggest that the fragment from the 3' end of the viral genome and/or possibly one or more internal fragment(s) are recognized by the transferase. These observations might indicate that bacteriophage RNAs contain certain features probably present in all tRNAs and which are recognized by the transferase.

摘要

通过与商业蛇毒磷酸二酯酶孵育获得的噬菌体RNA R17、MS2和Qβ的片段成为大肠杆菌tRNA核苷酸转移酶的底物。在其对缺乏CCA的tRNA仍保持高度特异性的条件下，该转移酶会添加回CMP和AMP。结果表明，病毒基因组3'端的片段和/或可能的一个或多个内部片段被该转移酶识别。这些观察结果可能表明，噬菌体RNA包含某些可能存在于所有tRNA中且被该转移酶识别的特征。