植物病毒RNA与tRNA核苷酸转移酶的相互作用

Interactions of plant viral RNAs and tRNA nucleotidyl transferase.

作者信息

Busto P, Carriquiry E, Tarrago-Litvak L, Castroviejo M, Litvak S

出版信息

Ann Microbiol (Paris). 1976 Jan;127A(1):39-46.

Abstract

Ribonucleic acid from TMV and BMV can accept AMP and CMP when digested with VPD. This incorporation is catalyzed by E. coli and yeast tRNA nucleotidyl transferases. Complex formation is obtained between TYMV RNA and tRNA nucleotidyl transferase in sucrose gradients while TMV and BMV RNAs failed to form a complex in the same conditions. The affinity of the enzyme for viral RNAs is lower than the affinity for tRNA as shown by complex formation on nitrocellulose filters and competition with tRNA. Coat protein from TMV particles enhances AMP and CMP incorporation onto tRNA catalyzed by the E. coli tRNA nucleotidyl transferase.

摘要

用VPD消化时，来自烟草花叶病毒（TMV）和黄瓜花叶病毒（BMV）的核糖核酸可以接受AMP和CMP。这种掺入由大肠杆菌和酵母tRNA核苷酸转移酶催化。在蔗糖梯度中，芜菁黄花叶病毒（TYMV）RNA与tRNA核苷酸转移酶之间形成复合物，而TMV和BMV RNA在相同条件下未能形成复合物。如在硝酸纤维素滤膜上形成复合物以及与tRNA竞争所示，该酶对病毒RNA的亲和力低于对tRNA的亲和力。TMV颗粒的衣壳蛋白增强了大肠杆菌tRNA核苷酸转移酶催化的AMP和CMP掺入tRNA的过程。