大鼠外周神经切断后脊髓反射易化的肽能介导可塑性

Plasticity of the peptidergic mediation of spinal reflex facilitation after peripheral nerve section in the rat.

作者信息

Wiesenfeld-Hallin Z, Xu X J, Håkanson R, Feng D M, Folkers K

机构信息

Department of Clinical Neurophysiology, Karolinska Institute, Huddinge University Hospital, Sweden.

出版信息

Neurosci Lett. 1990 Aug 24;116(3):293-8. doi: 10.1016/0304-3940(90)90089-r.

DOI:10.1016/0304-3940(90)90089-r

PMID:1700843

Abstract

The effects of the tachykinin antagonist Spantide II (D-Nic-Lys1,3-Pal3,D-Cl2Phe5,Asn6,D-Trp7,9,Nl e11)-substance P (SP) and the vasoactive intestinal peptide (VIP) antagonist (Ac-Tyr1,D-Phe2)-GRF(1-29)-NH2 on the excitability of the spinal nociceptive flexor reflex to intrathecally (i.t.) applied SP and VIP, respectively, as well as the facilitation evoked by activation of cutaneous C-afferent was examined. Both antagonists blocked the effects of the respective neuropeptides in rats with both intact and sectioned sciatic nerves. Spantide II antagonised C-afferent induced reflex facilitation in rats with intact nerves, but the degree of antagonism declined after axotomy. In contrast, the VIP antagonist did not block C-afferent induced facilitation in rats with intact nerves, but did so after axotomy. The results indicate that the role of tachykinins in mediating C-afferent-induced reflex facilitation is taken over by VIP after axotomy.

摘要

速激肽拮抗剂Spantide II（D-异亮氨酸-赖氨酸1,3-棕榈酰3，D-二氯苯丙氨酸5，天冬酰胺6，D-色氨酸7,9，Nle11）-P物质（SP）和血管活性肠肽（VIP）拮抗剂（Ac-酪氨酸1，D-苯丙氨酸2）-GRF（1-29）-NH2分别对鞘内（i.t.）注射SP和VIP时脊髓伤害性屈肌反射兴奋性的影响，以及对皮肤C类传入纤维激活所诱发的易化作用进行了研究。两种拮抗剂均能阻断完整和切断坐骨神经大鼠中各自神经肽的作用。Spantide II能拮抗完整神经大鼠中C类传入纤维诱发的反射易化作用，但在轴突切断后拮抗程度下降。相反，VIP拮抗剂在完整神经大鼠中不能阻断C类传入纤维诱发的易化作用，但在轴突切断后可以。结果表明，轴突切断后，速激肽在介导C类传入纤维诱发的反射易化作用中的作用被VIP取代。