Zheng Fang, Xu Luo, Verbiest Lieve, Verbeken Eric, De Ridder Dirk, Deprest Jan
Center for Surgical Technologies, University Hospitals Leuven, Katholieke Universiteit Leuven, Minderbroedersstraat 17, 3000-Leuven, Belgium.
Neurourol Urodyn. 2007;26(2):280-9. doi: 10.1002/nau.20317.
We earlier showed that xenogenic Pelvicol (Bard, Olen, Belgium) implants induce a lesser inflammatory response than Prolene (Johnson and Johnson, Dilbeek, Belgium). The purpose of this study was to determine cytokine profiles in the host immune responses to Pelvicol in a mouse model. The hypothesis was that Pelvicol would induce a "T-helper2" (Th2) rather than T-helper1 (Th1) type of inflammatory response.
Mice were implanted subcutaneously with Pelvicol or Prolene and the graft sites were harvested at 3 to 28 days. Histopathology was done and cytokine levels were determined by immunohistochemistry and RT-PCR. Flow cytometry was used to identify which cell population contributed to the observed cytokine production profiles.
Pelvicol induced a decreased inflammation and displayed an increase in IL-10 and TGF-beta, but reduce of TNF-alpha and IFN-gamma, indicating a Th2 type dominated response as examined by immunohistochemistry and RT-PCR. Flow cytometry showed that the monocytes/maceophages were the main cell population responsible for production of these cytokines. Monocytes/maceophages from Pelvicol explants showed upregulated expression of IL-10 while Prolene explants expressed TNF-alpha.
Pelvicol induced a Th2 type cytokine-dominated immune response after subcutaneous implantation in mice.
我们先前表明，异种Pelvicol（巴德公司，奥伦，比利时）植入物引起的炎症反应比普理灵（强生公司，迪尔贝克，比利时）更小。本研究的目的是在小鼠模型中确定宿主对Pelvicol免疫反应中的细胞因子谱。假设是Pelvicol会诱导“辅助性T细胞2型”（Th2）而非辅助性T细胞1型（Th1）的炎症反应。
将Pelvicol或普理灵皮下植入小鼠体内，在3至28天收获移植部位。进行组织病理学检查，并通过免疫组织化学和逆转录聚合酶链反应测定细胞因子水平。使用流式细胞术确定哪些细胞群促成了观察到的细胞因子产生谱。
Pelvicol诱导炎症减轻，白细胞介素-10（IL-10）和转化生长因子-β（TGF-β）增加，但肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）减少，免疫组织化学和逆转录聚合酶链反应检测表明这是一种以Th2型为主的反应。流式细胞术显示单核细胞/巨噬细胞是产生这些细胞因子的主要细胞群。来自Pelvicol外植体的单核细胞/巨噬细胞显示IL-10表达上调，而普理灵外植体表达TNF-α。
Pelvicol皮下植入小鼠后诱导了以Th2型细胞因子为主的免疫反应。
