新型免疫抑制剂FK506对加速型肾毒性血清性肾小球肾炎大鼠肾小球损伤的影响。

The effects of a new immunosuppressive agent, FK506, on the glomerular injury in rats with accelerated nephrotoxic serum glomerulonephritis.

作者信息

Hara S, Fukatsu A, Suzuki N, Sakamoto N, Matsuo S

机构信息

Third Department of Internal Medicine, Nagoya University School of Medicine, Japan.

出版信息

Clin Immunol Immunopathol. 1990 Dec;57(3):351-62. doi: 10.1016/0090-1229(90)90110-c.

DOI:10.1016/0090-1229(90)90110-c

PMID:1700936

Abstract

The aim of the present work was to study the effects of a new immunosuppressive drug, FK506, in accelerated nephrotoxic serum glomerulonephritis. Glomerulonephritis was induced in female Wistar rats by the preimmunization with normal rabbit IgG (Day-4) and the subsequent intravenous injection of rabbit anti-GBM serum (Day 0). Without treatment with FK506, rats developed proteinuria at Day 6 and onward. Rat anti-rabbit IgG was strongly detected at Day 6 and the titer was maintained through Day 20. Moderate hypercellularity and focal crescent formation were observed at Day 20. Rats injected intramuscularly with 0.3 or 1 mg/kg of FK506 did not develop proteinuria and the anti-rabbit IgG titer was much less or was undetectable throughout the experiments. These data suggest that FK506 is effective in the present model of glomerulonephritis.

摘要

本研究的目的是探讨新型免疫抑制药物FK506对加速型肾毒性血清性肾小球肾炎的影响。通过预先用正常兔IgG免疫雌性Wistar大鼠（第-4天），随后静脉注射兔抗GBM血清（第0天）来诱导肾小球肾炎。未经FK506治疗的大鼠在第6天及以后出现蛋白尿。在第6天强烈检测到大鼠抗兔IgG，并且其滴度在第20天一直维持。在第20天观察到中度细胞增多和局灶性新月体形成。肌肉注射0.3或1mg/kg FK506的大鼠未出现蛋白尿，并且在整个实验过程中抗兔IgG滴度低得多或无法检测到。这些数据表明FK506在本肾小球肾炎模型中是有效的。