FitzGerald P G, Casselman J
Department of Human Anatomy, School of Medicine, University of California, Davis 95616.
Curr Eye Res. 1990 Sep;9(9):873-82. doi: 10.3109/02713689008999559.
The lens fiber cell cytoskeleton includes a protein with a relative molecular weight, by SDS PAGE, of 115 kD. This protein has been purported to be, or be related to alpha-actinin, a highly-conserved family of actin-binding cytoskeletal proteins common to many tissues across a wide phylogenetic range. In this report we assess the relationship between the 115 kd lens fiber cell protein and alpha-actinin. Assessment of relative molecular weight, immunologic cross-reactivity, and partial sequence analysis suggest that the 115 kD lens fiber cell cytoskeletal protein and alpha-actinin are either unrelated, or, at best, that the lens protein represents an unusually divergent isoform of the alpha-actin family of proteins. Immunochemical analysis of homogenates of bovine heart and red blood cells indicate that these tissues express a protein which is weakly cross-reactive with the lens 115 kD protein, but that this cross-reactive protein is not alpha-actinin.
晶状体纤维细胞细胞骨架包含一种通过SDS-PAGE测定相对分子量为115kD的蛋白质。据称该蛋白质是α-辅肌动蛋白，或与α-辅肌动蛋白相关，α-辅肌动蛋白是一类高度保守的肌动蛋白结合细胞骨架蛋白家族，在广泛的系统发育范围内许多组织中都很常见。在本报告中，我们评估了115kd晶状体纤维细胞蛋白与α-辅肌动蛋白之间的关系。对相对分子量、免疫交叉反应性和部分序列分析的评估表明，115kD晶状体纤维细胞细胞骨架蛋白与α-辅肌动蛋白要么不相关，或者充其量，晶状体蛋白代表α-肌动蛋白家族蛋白质的一种异常分化的同种型。对牛心脏和红细胞匀浆的免疫化学分析表明，这些组织表达一种与晶状体115kD蛋白弱交叉反应的蛋白质，但这种交叉反应蛋白不是α-辅肌动蛋白。
