Kuhnert Yvonne, Schmäschke Ronald, Daugschies Arwid
Institut für Parasitologie, Veterinärmedizinische Fakultät, Universität Leipzig.
Berl Munch Tierarztl Wochenschr. 2006 Jul-Aug;119(7-8):282-6.
A highly sensitive diagnostic method is of great importance for detection of I. suis (Isospora suis). In the present study KSFV (combined sedimentation-flotation method) using different flotation solutions (NaCl, ZnSO4, NaCl-sucrose) was investigated regarding sensitivity, costs and expenditure of human labour in comparison to FV (flotation method) using NaCl-sucrose solution and to AM (autofluorescence microscopy). Ninety faecal samples were examined. Using AM, oocysts were detected in 83.3% of the samples. In cases of KSFV and FV, respectively, 41.1% to 53.3% of samples were tested positive. The quantity of detected oocysts was markedly higher in AM than in KSFV or FV. Therefore AM is the most sensitive of the comprised methods. The expenditure of labour is lowest in AM, time exposure is lower in FV than in KSFV. For performance of AM a fluorescence microscope is required contrary to KSFV and FV.
一种高灵敏度的诊断方法对于检测猪等孢球虫(Isospora suis)至关重要。在本研究中,针对使用不同浮选溶液(氯化钠、硫酸锌、氯化钠 - 蔗糖)的KSFV(沉降 - 浮选联合法),就其与使用氯化钠 - 蔗糖溶液的FV(浮选法)以及AM(自发荧光显微镜检查法)相比的灵敏度、成本和人力消耗进行了研究。检查了90份粪便样本。使用AM法时,在83.3%的样本中检测到了卵囊。在KSFV和FV法中,分别有41.1%至53.3%的样本检测呈阳性。AM法检测到的卵囊数量明显高于KSFV或FV法。因此,AM法是所包含方法中最灵敏的。AM法的人力消耗最低,FV法的检测时间比KSFV法短。与KSFV和FV法不同,AM法需要使用荧光显微镜。