Valer Karina, Fliegmann Judith, Fröhlich Andreas, Tyler Brett M, Ebel Jürgen
Department Biologie I-Botanik, Ludwig-Maximilians-Universität, Menzinger Strasse 67, D-80638 Munich, Germany.
FEMS Microbiol Lett. 2006 Dec;265(1):60-8. doi: 10.1111/j.1574-6968.2006.00467.x. Epub 2006 Sep 29.
The Avr1b locus is required for avirulence of the oomycete pathogen Phytophthora sojae on soybeans carrying resistance gene Rps1b. One of the Avr genes of the locus (Avr1b-1) was shown to encode an elicitor. We have analyzed the spatial and temporal expression patterns of Avr1b-1 in comparison to defense-related genes induced in soybean. Avr1b-1 expression was detectable mainly in close proximity to the site of infection, in wound-inoculated hypocotyls as well as in roots infected with zoospores. Usually, in compatible interactions, higher expression levels of Avr1b-1 were observed in roots when compared with incompatible P. sojae-soybean interactions, whereas neither the timing nor the amount of transcript accumulation of defense-related genes showed cultivar-specific differences. In contrast, the PsojNIP gene encoding a proposed virulence factor was expressed only during the necrotrophic phase in the compatible interaction.
Avr1b基因座对于卵菌病原体大豆疫霉在携带抗性基因Rps1b的大豆上的无毒力是必需的。该基因座的一个Avr基因（Avr1b-1）已被证明可编码一种激发子。我们分析了Avr1b-1与大豆中诱导的防御相关基因相比的时空表达模式。Avr1b-1的表达主要在感染部位附近、伤口接种的下胚轴以及游动孢子感染的根中可检测到。通常，在亲和互作中，与不亲和的大豆疫霉-大豆互作相比，在根中观察到Avr1b-1的表达水平更高，而防御相关基因转录本积累的时间和量均未显示出品种特异性差异。相反，编码一种假定毒力因子的PsojNIP基因仅在亲和互作的坏死营养阶段表达。
