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属于C/EBP家族成员的转录因子AGP/EBP的分子克隆。

Molecular cloning of a transcription factor, AGP/EBP, that belongs to members of the C/EBP family.

作者信息

Chang C J, Chen T T, Lei H Y, Chen D S, Lee S C

机构信息

Institute of Biological Chemistry, Academia Sinica, Taiwan.

出版信息

Mol Cell Biol. 1990 Dec;10(12):6642-53. doi: 10.1128/mcb.10.12.6642-6653.1990.

DOI:10.1128/mcb.10.12.6642-6653.1990
PMID:1701020
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC362942/
Abstract

A C/EBP-like transcription factor, AGP/EBP, that binds to three distinct motifs in the 5'-flanking region of alpha 1-acid glycoprotein gene (AGP) has been identified. Here we report the cloning and properties of cDNA corresponding to mouse AGP/EBP. AGP/EBP and C/EBP share 87% amino acid sequence homology in the "leucine zipper" and its associated DNA-binding domains, while their sequences outside these domains and the sizes of their mRNAs are different. Unlike the limited expression of C/EBP in tissues and cells, AGP/EBP appears to be ubiquitously expressed in tissues like lung, spleen, kidney, heart, testis, and liver and cell lines like p388D1, 129P (hepatoma cell line of C3H/HeJ), FO (mouse myeloma), and L929. Antibody against cloned and expressed AGP/EBP which was raised in rabbits could recognize AGP/EBP from nuclear extract of a number of cells and tissues. On the basis of our findings about the structural relationship and the similarity of motif recognition, we propose that a family of C/EBP-like transcription factors exists.

摘要

已鉴定出一种与C/EBP相似的转录因子AGP/EBP，它能与α1-酸性糖蛋白基因（AGP）5'-侧翼区域的三个不同基序结合。在此，我们报告了与小鼠AGP/EBP相对应的cDNA的克隆及特性。AGP/EBP与C/EBP在“亮氨酸拉链”及其相关的DNA结合结构域中具有87%的氨基酸序列同源性，而它们在这些结构域之外的序列以及mRNA的大小是不同的。与C/EBP在组织和细胞中的有限表达不同，AGP/EBP似乎在肺、脾、肾、心脏、睾丸和肝脏等组织以及p388D1、129P（C3H/HeJ肝癌细胞系）、FO（小鼠骨髓瘤）和L929等细胞系中普遍表达。用兔制备的针对克隆并表达的AGP/EBP的抗体能够识别许多细胞和组织核提取物中的AGP/EBP。基于我们对结构关系和基序识别相似性的研究结果，我们提出存在一个C/EBP样转录因子家族。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5200/362942/a429bf7f12a3/molcellb00048-0563-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5200/362942/3fb5f8f6afa2/molcellb00048-0556-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5200/362942/a980cd4fdce0/molcellb00048-0556-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5200/362942/182b7b807fbe/molcellb00048-0557-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5200/362942/47d4af8c73bf/molcellb00048-0559-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5200/362942/1a76788fd80d/molcellb00048-0559-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5200/362942/5f3c02c00c97/molcellb00048-0561-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5200/362942/fa914ab6ca10/molcellb00048-0561-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5200/362942/181f0014316c/molcellb00048-0562-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5200/362942/a429bf7f12a3/molcellb00048-0563-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5200/362942/3fb5f8f6afa2/molcellb00048-0556-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5200/362942/a980cd4fdce0/molcellb00048-0556-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5200/362942/182b7b807fbe/molcellb00048-0557-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5200/362942/47d4af8c73bf/molcellb00048-0559-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5200/362942/1a76788fd80d/molcellb00048-0559-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5200/362942/5f3c02c00c97/molcellb00048-0561-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5200/362942/fa914ab6ca10/molcellb00048-0561-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5200/362942/181f0014316c/molcellb00048-0562-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5200/362942/a429bf7f12a3/molcellb00048-0563-a.jpg

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