Henriksson R, Sundström S, Danielsson A, Hellström S
Department of Histology, University of Umeå, Sweden.
Acta Physiol Scand. 1990 Aug;139(4):597-601. doi: 10.1111/j.1748-1716.1990.tb08964.x.
Unilateral sympathetic denervation of rat parotid glands was performed within 4 h after birth. Nine weeks later the glands were used for in-vitro studies of amylase secretion, and 86Rb+ was used as a marker for potassium efflux. The non-denervated contralateral glands served as controls. The tissue concentrations of 5-hydroxytryptamine and its metabolite 5-hydroxyindole acetic acid were also measured. 5-Hydroxytryptamine caused a significant dose-dependent increase in amylase secretion, which was inhibited by methysergide. There was no difference between controls and denervated glands. 5-Hydroxytryptamine was without effect on potassium efflux from either denervated or control glands. The sympathectomy caused increased levels of 5-hydroxytryptamine and 5-hydroxyindole acetic acid as compared with contralateral controls. The results suggest that 5-hydroxytryptamine influences the two main secretory processes in rat parotid gland differently. A significant amylase discharge was seen following 5-hydroxytryptamine stimulation, whereas no effect was seen on 86Rb+ efflux. Although it is also proposed that there are no 5-hydroxytryptamine-associated nerves in the superior cervical ganglion innervating parotid tissue, it seems that there is a complex connection between the sympathetic pathway and the serotoninergic system.
在大鼠出生后4小时内对其腮腺进行单侧交感神经切除术。9周后，将这些腺体用于淀粉酶分泌的体外研究，并使用86Rb+作为钾外流的标志物。未去神经支配的对侧腺体作为对照。还测量了5-羟色胺及其代谢产物5-羟吲哚乙酸的组织浓度。5-羟色胺导致淀粉酶分泌显著的剂量依赖性增加，这一作用被麦角新碱抑制。去神经支配的腺体与对照腺体之间没有差异。5-羟色胺对去神经支配的腺体或对照腺体的钾外流均无影响。与对侧对照相比，交感神经切除术导致5-羟色胺和5-羟吲哚乙酸水平升高。结果表明，5-羟色胺对大鼠腮腺的两个主要分泌过程的影响不同。5-羟色胺刺激后可见显著的淀粉酶释放，而对86Rb+外流无影响。尽管也有人提出，支配腮腺组织的颈上神经节中不存在与5-羟色胺相关的神经，但交感神经通路与5-羟色胺能系统之间似乎存在复杂的联系。
