一种对结核分枝杆菌有反应的单克隆抗体（P6）的制备与特性分析。

Production and characterization of a monoclonal antibody (P6) reactive to Mycobacterium tuberculosis.

作者信息

Kapoor J, Batra H V, Gupta S K, Moudgil K D, Talwar G P

机构信息

National Institute of Immunology, JNU Campus, New Delhi, India.

出版信息

Zentralbl Bakteriol. 1990 Sep;273(4):545-54. doi: 10.1016/s0934-8840(11)80462-7.

DOI:10.1016/s0934-8840(11)80462-7

PMID:1701087

Abstract

The production and characterization of a murine monoclonal antibody (P6) to Mycobacterium tuberculosis is described. The specificity of the monoclonal antibody, an IgG1/K type, was tested against sonic extracts obtained from 27 mycobacteria and 39 bacteria by an enzyme-linked immunosorbent assay. Apparently it was highly specific for the virulent form of M. tuberculosis (H37Rv) and did not react with avirulent strains of M. tuberculosis (H37Ra) and M. bovis (BCG). It was devoid of reactivity with all other mycobacteria and bacteria except to a reaction of low extent with Nocardia asteroides (11.8%). The antibody recognized intact M. tuberculosis as well as the sonicate. Various pathogenic strains of M. tuberculosis collected from different geographical locations showed a range of reactivity with this monoclonal antibody. The antibody reacted strongly with M. tuberculosis South Indian (79112) and British strain (wild) and to varying extents with other strains of M. tuberculosis tested. In Western immunoblots, it recognized antigens of 45 and 96 KDa of M. tuberculosis (H37Rv).

摘要

本文描述了一种针对结核分枝杆菌的鼠单克隆抗体（P6）的制备及特性。通过酶联免疫吸附测定法，对该IgG1/K型单克隆抗体的特异性进行了检测，检测对象为从27种分枝杆菌和39种细菌中提取的超声提取物。显然，它对结核分枝杆菌的强毒株（H37Rv）具有高度特异性，而不与结核分枝杆菌无毒株（H37Ra）和牛分枝杆菌（卡介苗）发生反应。除了与星形诺卡菌有低程度反应（11.8%）外，它与所有其他分枝杆菌和细菌均无反应。该抗体可识别完整的结核分枝杆菌以及超声提取物。从不同地理位置收集的各种结核分枝杆菌致病菌株与该单克隆抗体呈现出一系列反应。该抗体与南印度结核分枝杆菌（79112）和英国菌株（野生型）反应强烈，与其他测试的结核分枝杆菌菌株反应程度各异。在Western免疫印迹中，它识别结核分枝杆菌（H37Rv）的45 kDa和96 kDa抗原。