Nanba Daisuke, Kinugasa Yumi, Morimoto Chie, Koizumi Michiko, Yamamura Hisako, Takahashi Katsuhito, Takakura Nobuyuki, Mekada Eisuke, Hashimoto Koji, Higashiyama Shigeki
Department of Biochemistry and Molecular Genetics, Ehime University Graduate School of Medicine, Shitsukawa, To-on, Ehime 791-0295, Japan.
Biochem Biophys Res Commun. 2006 Nov 17;350(2):315-21. doi: 10.1016/j.bbrc.2006.09.060. Epub 2006 Sep 22.
Epidermal growth factor (EGF) and ErbB family molecules play a role in heart development and function. To investigate the role of EGF family member, heparin-binding EGF-like growth factor (HB-EGF) in heart development, smooth muscle and endothelial cell lineage-specific HB-EGF knockout mice were generated using the Cre/loxP system in combination with the SM22alpha or TIE2 promoter. HB-EGF knockout mice displayed enlarged heart valves, and over half of these mice died during the first postnatal week, while survivors showed cardiac hypertrophy. These results suggest that expression of HB-EGF in smooth muscle and/or endothelial cell lineages is essential for proper heart development and function in mice.
表皮生长因子（EGF）和ErbB家族分子在心脏发育和功能中发挥作用。为了研究EGF家族成员肝素结合表皮生长因子样生长因子（HB-EGF）在心脏发育中的作用，利用Cre/loxP系统结合SM22α或TIE2启动子构建了平滑肌和内皮细胞谱系特异性HB-EGF基因敲除小鼠。HB-EGF基因敲除小鼠表现出心脏瓣膜增大，超过半数的小鼠在出生后第一周死亡，而存活的小鼠表现出心脏肥大。这些结果表明，平滑肌和/或内皮细胞谱系中HB-EGF的表达对于小鼠正常的心脏发育和功能至关重要。
