用于人群研究的尿β-促黄体生成素和β-促卵泡生成素免疫酶分析法。

Urinary beta-luteinizing hormone and beta-follicle stimulating hormone immunoenzymometric assays for population research.

作者信息

Brindle Eleanor, Miller Rebecca C, Shofer Jane B, Klein Nancy A, Soules Michael R, O'Connor Kathleen A

机构信息

Center for Studies in Demography and Ecology, Box 353412, University of Washington, Seattle, Washington 98195, USA.

出版信息

Clin Biochem. 2006 Nov;39(11):1071-9. doi: 10.1016/j.clinbiochem.2006.08.009. Epub 2006 Sep 1.

OBJECTIVE

We developed assays for measurement of urinary betaLH and betaFSH under collection and storage conditions typical of non-clinical research settings.

DESIGN AND METHODS

IEMAs for free betaLH and total betaFSH were validated by standard methods. Stability of urinary betaLH and betaFSH was tested across freeze-thaws and stored long term at 4 degrees C or -20 degrees C, or short term at room temperature, and with heating to dissociate the subunits.

RESULTS

The IEMAs exhibited acceptable parallelism, specificity, recovery (averaging 100% for betaLH, 97% for betaFSH), imprecision (maximum within-run and between run CVs, respectively, 4.8% and 25.7% for betaLH, 5.6% and 17.0% for betaFSH), and minimum detectable dose (2.5 pmol/L for betaLH, 6.8 pmol/L for betaFSH). Urine and serum measures were highly correlated (r=0.95 for LH, 0.86 for FSH). There was no consistent decline with any storage type. Dissociation of subunits by heating was needed for betaLH, but not betaFSH.

CONCLUSION

These IEMAs measure free betaLH and total betaFSH, overcoming inter-individual variability in, and collection and storage effects on, subunit dissociation, without the need for urine preservatives.

目的

我们开发了在非临床研究环境典型的收集和储存条件下测量尿β促黄体生成素（βLH）和β促卵泡生成素（βFSH）的检测方法。

设计与方法

采用标准方法对游离βLH和总βFSH的免疫酶联测定法（IEMAs）进行验证。在冻融过程中以及在4℃或-20℃长期储存、室温短期储存以及加热使亚基解离的条件下，测试尿βLH和βFSH的稳定性。

结果

免疫酶联测定法表现出可接受的平行性、特异性、回收率（βLH平均为100%，βFSH为97%）、不精密度（βLH批内和批间变异系数最大值分别为4.8%和25.7%，βFSH分别为5.6%和17.0%）以及最低检测剂量（βLH为2.5 pmol/L，βFSH为6.8 pmol/L）。尿和血清检测结果高度相关（LH的r = 0.95，FSH的r = 0.86）。任何储存类型均未出现一致的下降情况。βLH需要通过加热使亚基解离，而βFSH则不需要。