金属和洗涤剂对跳鲻（Liza saliens）生物转化和解毒酶的影响。
Effects of metals and detergents on biotransformation and detoxification enzymes of leaping mullet (Liza saliens).
作者信息
Sen Alaattin, Semiz Asli
机构信息
Department of Biology, Pamukkale University, Kinikli-Denizli 20017, Turkey.
出版信息
Ecotoxicol Environ Saf. 2007 Nov;68(3):405-11. doi: 10.1016/j.ecoenv.2006.08.007. Epub 2006 Oct 2.
In this study, feral leaping mullet (Liza saliens) liver microsomal 7-ethoxyresorufin O-deethylase (EROD), and cytosolic glutathione S-transferases (GSTs) activities were investigated using 7-ethoxyresorufin, 1-chloro-2,4-dinitrobenzene (CDNB), and ethacrynic acid (EA) as substrates, respectively. The average EROD activity was found as 1139+/-175 pmol resorufin/min/mg protein. The average GST activities towards CDNB and EA were found as 1364+/-41 and 140+/-19 nmol/min/mg protein, respectively. We have, then, investigated the in vitro effects of some metals and detergents on CYP1A and GST activities in leaping mullet liver. Leaping mullet liver microsomal EROD activity was significantly inhibited by Hg (0.1 mM), Ni (0.1 mM), Cd (0.1 mM), Cu (0.1 mM), Zn (0.1 mM), Sb (0.1 mM), Fe2+ (1 mM), Co (1 mM), Al (1 mM), and Fe3+ (1 mM), with the percent inhibition of 80, 80, 77, 75, 70, 69, 56, 53, 46, and 44, respectively. Similarly, conjugation of CDNB catalyzed by GST was inhibited significantly to lesser extend by Hg (0.1mM), Sb (0.1 mM), Cd (0.1 mM), Cu (0.1 mM), Zn (0.1 mM), Fe3+ (1 mM), Co (1 mM), and Fe2+ (1 mM), with the percent inhibition of 70, 69, 65, 61, 54, 51, 47, and 43, respectively. The degrees of inhibition observed on GST catalyzed EA conjugation by Hg (0.1 mM), Cd (0.1 mM), Sb (0.1 mM), Cu (0.1 mM), and Zn (0.1 mM) were 86, 78, 69, 51, and 42, respectively. In addition to metals, the effect of various detergents on leaping mullet liver EROD, GST-CDNB, and GST-EA activities were studied. It was found that ionic detergents strongly inhibited the EROD activity, whereas much less inhibitions were observed with GST catalyzed activities. Therefore, the CYP1A inhibition potencies of metals and detergents suggest that their contribution to the overall CYP1A induction in polycyclic aromatic hydrocarbons contaminated environmental samples has to be taken into account for better interpretation of environmental studies.
在本研究中，分别以7 - 乙氧基试卤灵、1 - 氯 - 2,4 - 二硝基苯（CDNB）和依他尼酸（EA）为底物，研究了野生跳弹涂鱼（Liza saliens）肝脏微粒体7 - 乙氧基试卤灵O - 脱乙基酶（EROD）和胞质谷胱甘肽S - 转移酶（GSTs）的活性。发现EROD的平均活性为1139±175 pmol试卤灵/分钟/毫克蛋白。对CDNB和EA的GST平均活性分别为1364±41和140±19 nmol/分钟/毫克蛋白。然后，我们研究了一些金属和洗涤剂对跳弹涂鱼肝脏中CYP1A和GST活性的体外影响。Hg（0.1 mM）、Ni（0.1 mM）、Cd（0.1 mM）、Cu（0.1 mM）、Zn（0.1 mM）、Sb（0.1 mM）、Fe2 +（1 mM）、Co（1 mM）、Al（1 mM）和Fe3 +（1 mM）显著抑制了跳弹涂鱼肝脏微粒体EROD活性，抑制率分别为80%、80%、77%、75%、70%、69%、56%、53%、46%和44%。同样，Hg（0.1 mM）、Sb（0.1 mM）、Cd（0.1 mM）、Cu（0.1 mM）、Zn（0.1 mM）、Fe3 +（1 mM）、Co（1 mM）和Fe2 +（1 mM）对GST催化的CDNB结合反应有不同程度的显著抑制，抑制率分别为70%、69%、65%、61%、54%、51%、47%和43%。Hg（0.1 mM）、Cd（0.1 mM）、Sb（0.1 mM）、Cu（0.1 mM）和Zn（0.1 mM）对GST催化的EA结合反应的抑制程度分别为86%、78%、69%、51%和42%。除金属外，还研究了各种洗涤剂对跳弹涂鱼肝脏EROD、GST - CDNB和GST - EA活性的影响。发现离子型洗涤剂强烈抑制EROD活性，而对GST催化的活性抑制作用较小。因此，金属和洗涤剂对CYP1A的抑制作用表明，在解释环境研究结果时，必须考虑它们对多环芳烃污染环境样品中整体CYP1A诱导的贡献。
Zotero 插件