NADPH氧化酶调节亚基p40phox的结晶及初步晶体学分析
Crystallization and preliminary crystallographic analysis of p40phox, a regulatory subunit of NADPH oxidase.
作者信息
Honbou Kazuya, Yuzawa Satoru, Suzuki Nobuo N, Fujioka Yuko, Sumimoto Hideki, Inagaki Fuyuhiko
机构信息
Department of Structural Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University, N-12, W-6, Kita-ku, Sapporo 060-0812, Japan.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Oct 1;62(Pt 10):1018-20. doi: 10.1107/S1744309106036256. Epub 2006 Sep 30.
Abstract
p40(phox) is a cytosolic component of the phagocyte NADPH oxidase, which is responsible for production of the superoxide that kills invasive microorganisms. Full-length p40(phox) was expressed in Escherichia coli, purified and crystallized by the sitting-drop vapour-diffusion method at 293 K using polyethylene glycol 20,000 as a precipitant. Diffraction data were collected to 3.0 A resolution at 100 K using synchrotron radiation. The crystal belongs to space group C222(1), with unit-cell parameters a = 146.27, b = 189.81, c = 79.88 A. This crystal was estimated to contain two or three protein molecules per asymmetric unit from the acceptable range of volume-to-weight ratio values.
摘要
p40(phox)是吞噬细胞NADPH氧化酶的一种胞质成分，该氧化酶负责产生杀死入侵微生物的超氧化物。全长p40(phox)在大肠杆菌中表达，经纯化后，于293 K采用坐滴气相扩散法，以聚乙二醇20000作为沉淀剂进行结晶。利用同步辐射在100 K下收集到分辨率为3.0 Å的衍射数据。晶体属于空间群C222(1)，晶胞参数a = 146.27、b = 189.81、c = 79.88 Å。根据体积与重量比值的可接受范围估计，该晶体每个不对称单位包含两个或三个蛋白质分子。
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1
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2
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3
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J Biol Chem. 2005 Apr 8;280(14):13752-61. doi: 10.1074/jbc.M412897200. Epub 2005 Jan 18.
4
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J Biol Chem. 2004 Jun 18;279(25):26378-86. doi: 10.1074/jbc.M309724200. Epub 2004 Apr 21.
5
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Mol Cell. 2003 Jul;12(1):39-50. doi: 10.1016/s1097-2765(03)00246-6.
6
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EMBO J. 2002 Dec 2;21(23):6312-20. doi: 10.1093/emboj/cdf642.
7
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8
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9
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