Miyamoto Hiroyuki, Tazawa Yutaka, Hayasaka Akira, Nitta Junfuku, Egawa Isao, Kurosaka Daijiro
Department of Ophthalmology, Iwate Medical University School of Medicine, Morioka, Japan.
Jpn J Ophthalmol. 2006 Sep-Oct;50(5):432-437. doi: 10.1007/s10384-006-0345-x.
To characterize the s-wave of the multifocal electroretinogram (mfERG) in cats, and to determine the contribution of the inner retina to the s-wave by examining the effects of tetrodotoxin (TTX) and N-methyl D: -aspartate (NMDA) injected into the vitreous cavity.
mfERGs were recorded from 15 eyes of 15 male cats under general anesthesia. The stimulus consisted of 37 elements, and the luminance of the bright and the black elements were 200 and 4 cd/m2, respectively. The stimuli were presented in a pseudorandom binary m-sequence at six different base periods (bpds) from 13.3 to 426.7 ms. Fifty microliters of 7.0 microM TTX followed by 50 microl of 4.0 mM NMDA were injected into the vitreous cavity.
The shape of the mfERGs in the cats resembled that in humans. The s-wave appeared on the descending limb of P1, as seen in human mfERGs, in 11 eyes, and the s-wave amplitude increased significantly as the bpd was increased. TTX and NMDA resulted in the disappearance of the s-wave at all bpds, while the amplitude of P1 remained unchanged.
The s-wave is present in the mfERG in the cat, and its loss following injections of TTX and NMDA supports the view that the s-wave reflects the function of the ganglion cells and their axons.
表征猫多焦视网膜电图（mfERG）的s波，并通过检查注入玻璃体腔的河豚毒素（TTX）和N-甲基-D-天冬氨酸（NMDA）的作用来确定内层视网膜对s波的贡献。
在全身麻醉下从15只雄性猫的15只眼睛记录mfERG。刺激由37个单元组成，亮单元和黑单元的亮度分别为200和4 cd/m²。刺激以伪随机二进制m序列在6个不同的基础周期（bpds）从13.3到426.7 ms呈现。将50微升7.0微摩尔/升的TTX，随后50微升4.0毫摩尔/升的NMDA注入玻璃体腔。
猫的mfERG形状与人类相似。如在人类mfERG中所见，11只眼中s波出现在P1的下降支上，并且随着bpd增加s波振幅显著增加。TTX和NMDA导致所有bpds下s波消失，而P1的振幅保持不变。
猫的mfERG中存在s波，注射TTX和NMDA后s波消失支持了s波反映神经节细胞及其轴突功能的观点。
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