Department of Ophthalmology, Glostrup Hospital, University of Copenhagen, Copenhagen, Denmark.
Graefes Arch Clin Exp Ophthalmol. 2012 Jan;250(1):79-86. doi: 10.1007/s00417-011-1782-x. Epub 2011 Aug 26.
The functional consequence of long-term retinal detachment in the porcine model is examined by multifocal electroretinography (mfERG). Retinal detachment (RD) in humans leaves permanent visual impairment, despite anatomical successful reattachment surgery. To improve treatment, adjuvant pharmaceutical therapy is needed, and can only be tested in a suitable animal model. The porcine model is promising and the mfERG is well validated in this model. RD was induced in 18 pigs by vitrectomy and healon injection of various concentrations. Preoperatively and 6 weeks postoperatively eight animals were examined by mfERG. The major component P1 was analyzed statistically. Indirect ophthalmoscopy and bilateral color fundus photography (FP) were performed. Selected animals underwent high-resolution optical coherence tomography (OCT). Examination by ophthalmoscopy and FP showed that the RDs remained detached for the 6 weeks of follow-up. The P1 amplitude of the mfERG did not differ significantly between the detached areas, the surrounding attached areas, and the healthy eye (p = 0.25). Similarly, P1 implicit time did not differ between the areas (p = 0.85). The lack of functional consequences of long-term RD makes the porcine model unsuitable for examining adjuvant pharmaceutical RD treatment. Future studies should focus on foveated primates.
通过多焦视网膜电图（mfERG）检查了猪模型中长期视网膜脱离的功能后果。尽管解剖学上的成功复位手术，但人类的视网膜脱离（RD）仍会导致永久性视力障碍。为了改善治疗效果，需要辅助药物治疗，而这种治疗只能在合适的动物模型中进行测试。猪模型很有前途，并且 mfERG 在该模型中得到了很好的验证。通过玻璃体切除术和不同浓度的海洛因注射在 18 头猪中诱导 RD。术前和术后 6 周，对 8 只动物进行 mfERG 检查。对主要成分 P1 进行了统计学分析。进行间接检眼镜检查和双侧眼底彩色照相术（FP）。选择的动物进行高分辨率光学相干断层扫描（OCT）。检眼镜和 FP 检查显示，在 6 周的随访中，RD 仍保持脱离状态。mfERG 的 P1 幅度在脱离区域、周围附着区域和健康眼之间没有显着差异（p = 0.25）。同样，区域之间的 P1 潜伏期也没有差异（p = 0.85）。长期 RD 没有产生功能后果，这使得猪模型不适合检查辅助药物 RD 治疗。未来的研究应集中在有中心凹的灵长类动物上。
