Clarke Matthew L, Chen Zhan
Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA.
Langmuir. 2006 Oct 10;22(21):8627-30. doi: 10.1021/la0607656.
Surface side-chain orientation changes of two polymers have been observed upon protein adsorption using sum frequency generation vibrational spectroscopy. Side-chain-deuterated poly(ethyl methacrylate) and poly(n-butyl methacrylate) were contacted with five protein solutions: albumin, fibrinogen, ubiquitin, cytochrome c, and lysozyme. The CD(3)/CD(2) symmetric stretch ratios of the surface polymer side chains in contact with these different media were compared to each other and to that of the polymer contacting air or phosphate buffered saline. The adsorption of different proteins to the surfaces resulted in polymer side-chain orientations slightly different from each other, with orientations between the air and buffer cases.
使用和频振动光谱法观察到，在蛋白质吸附过程中两种聚合物的表面侧链取向发生了变化。将侧链氘代的聚甲基丙烯酸乙酯和聚甲基丙烯酸正丁酯与五种蛋白质溶液接触：白蛋白、纤维蛋白原、泛素、细胞色素c和溶菌酶。将与这些不同介质接触的表面聚合物侧链的CD(3)/CD(2)对称拉伸比相互比较，并与接触空气或磷酸盐缓冲盐水的聚合物的该比值进行比较。不同蛋白质在表面的吸附导致聚合物侧链取向彼此略有不同，介于接触空气和缓冲液的情况之间。
