Ferreyra N F, Forzani E S, López Teijelo M, Coche-Guérente L, Labbé P
INFIQC-Departamento de Físicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000 Córdoba, Argentina.
Langmuir. 2006 Oct 10;22(21):8931-8. doi: 10.1021/la061173q.
The ellipsometric characterization of a layer-by-layer electrostatically self-assembled multilayer of polyphenol oxidase and alkaline phosphatase with the polycation poly(dimethyldiallylammonium chloride) built on an immunologic layer formed by immunoglobulin G (IgG) and glucose oxidase-conjugated anti-IgG (IgG-GOD) on glassy carbon is reported. The step-by-step evolution of the psi-Delta ellipsometric angles was followed during film growth. Two optical models, named the three-layer film model and reorganization film model, were employed and found suitable for ellipsometric data interpretation. A comparative analysis of film optical properties, film thickness, and ellipsometric mass assessed from both models is also presented.
报道了在玻碳上由免疫球蛋白G（IgG）和葡萄糖氧化酶偶联的抗IgG（IgG-GOD）形成的免疫层上构建的，由多酚氧化酶和碱性磷酸酶与聚阳离子聚（二甲基二烯丙基氯化铵）逐层静电自组装多层膜的椭偏表征。在膜生长过程中跟踪了ψ-Δ椭偏角的逐步演变。采用了两种光学模型，即三层膜模型和重组膜模型，发现它们适用于椭偏数据解释。还给出了从这两种模型评估的膜光学性质、膜厚度和椭偏质量的比较分析。
