Kador Peter F, Betts Daniel, Wyman Milton, Blessing Karen, Randazzo James
College of Pharmacy, University of Nebraska Medical Center, Omaha, NE 68198, Therapeutic Vision Inc, 11778 Whitmore S, Omaha, NE 68142, USA.
Am J Vet Res. 2006 Oct;67(10):1783-7. doi: 10.2460/ajvr.67.10.1783.
To determine effects of a topical formulation of an aldose reductase inhibitor (ARI) on the development of sugar cataracts in dogs fed a diet high in galactose. Animals-Ten 6-month old Beagles.
Dogs were fed a diet containing 30% galactose, and after 16 weeks, 6 dogs were treated topically with a proprietary ARI formulation and 4 dogs were treated with a placebo. Cataract formation was monitored by means of slit-lamp biomicroscopy and fundus photography. Dogs were euthanized after 10 weeks of treatment, and lenses were evaluated for degree of opacity, myo-inositol and galactitol concentrations, and concentration of the ARI.
All dogs developed bilateral cortical opacities dense enough to result in a decrease in the tapetal reflex after being fed the galactose-containing diet for 16 weeks. Administration of the ARI arrested further development of cataract formation. In contrast, cataracts in the vehicle-treated dogs progressed over the 10-week period to the mature stage. Evaluation of the isolated lenses after 26 weeks of galactose feeding indicated that lenses from treated dogs were significantly less optically dense than lenses from control dogs. Lenticular myo-inositol concentration was significantly higher in the treated than in the control dogs.
Results suggest that topical application of a proprietary ARI formulation may arrest or reverse the development of sugar cataracts in dogs fed a diet high in galactose. This suggests that this ARI formulation may be beneficial in maintaining or improving functional vision in diabetic dogs with early lens opacities.
确定一种醛糖还原酶抑制剂（ARI）局部制剂对喂食高半乳糖饮食的犬类糖性白内障发展的影响。动物——十只6个月大的比格犬。
给犬喂食含30%半乳糖的饮食，16周后，6只犬局部使用一种专利ARI制剂进行治疗，4只犬使用安慰剂进行治疗。通过裂隙灯生物显微镜检查和眼底摄影监测白内障的形成。治疗10周后对犬实施安乐死，并对晶状体的混浊程度、肌醇和半乳糖醇浓度以及ARI浓度进行评估。
所有犬在喂食含半乳糖饮食16周后均出现双侧皮质混浊，混浊程度足以导致反光减少。使用ARI可阻止白内障形成的进一步发展。相比之下，接受赋形剂治疗的犬的白内障在10周内发展到成熟阶段。在喂食半乳糖26周后对分离出的晶状体进行评估，结果表明，接受治疗的犬的晶状体的光学密度明显低于对照犬的晶状体。治疗组犬晶状体中的肌醇浓度明显高于对照组犬。
结果表明，局部应用一种专利ARI制剂可能阻止或逆转喂食高半乳糖饮食的犬类糖性白内障的发展。这表明该ARI制剂可能有助于维持或改善患有早期晶状体混浊的糖尿病犬的功能性视力。
