Lestage J, Bellott R, Metivaud J, Bon A, Giroud J P, Laulan A, Châteaureynaud P
UA 1136 CNRS, Université de Bordeaux I, Talence, France.
Hum Reprod. 1990 Aug;5(6):747-54. doi: 10.1093/oxfordjournals.humrep.a137180.
In 15 pregnant women during the third term of pregnancy, the immunomodulatory property of alpha 2-macroglobulin (alpha 2M) was initially detected by measuring the inhibitory effect on immune complement-dependent haemolysis of serum alpha 2M fractions obtained by gel filtration. By a two-step chromatography procedure consisting of gel filtration followed by anion-exchange chromatography, different sub-forms of alpha 2M in serum were separated. Amongst them, it was shown that the inhibition of complement activity was almost exclusively linked to one particular subform. Additional studies revealed that the observed effect was not due to proteases bound to alpha 2M during clotting since, by using protease-specific inhibitors, no change was observed in complement inhibition. This subform, though present at very low levels in control sera, appeared in strikingly increased amounts during the third trimester of pregnancy (35 mg/l) and comprised between 3 and 5% of the total alpha 2M. Results show that the increase of alpha 2M anticomplementary activity is linked to the increase in alpha 2M levels in serum.
在15名处于妊娠晚期的孕妇中，最初通过测量凝胶过滤获得的血清α2-巨球蛋白（α2M）组分对免疫补体依赖性溶血的抑制作用，检测了α2M的免疫调节特性。通过由凝胶过滤和阴离子交换色谱组成的两步色谱法，分离了血清中不同亚形式的α2M。其中，结果表明补体活性的抑制几乎完全与一种特定亚形式相关。进一步的研究表明，观察到的效应并非由于凝血过程中与α2M结合的蛋白酶所致，因为使用蛋白酶特异性抑制剂后，补体抑制作用未发生变化。这种亚形式虽然在对照血清中含量极低，但在妊娠晚期显著增加（35毫克/升），占总α2M的3%至5%。结果表明，α2M抗补体活性的增加与血清中α2M水平的升高有关。
