Silva E S
Instituto Nacional de Saude, Laboratório de Microbiologia Experimental, Lisboa, Portugal.
J Environ Pathol Toxicol Oncol. 1990 May-Jun;10(3):124-8.
Dinoflagellate blooms of the same species have been registered either as toxic or nontoxic and, in the latter case, toxicity may be of different types. A hypothesis has been formulated according to which the bacteria having in some way taken part in the toxin formation are either inside the dinoflagellate cell or in the nutritive liquid. The presence of intracellular bacteria in those microorganisms has been studied mainly in material from cultures, a few from the sea, and several strains were isolated from different species. Experiments with crossed inoculations have shown that the bacterial strain from Gonyaulax tamarensis caused the cells of some other species to become toxic. From nontoxic clonal cultures of Prorocentrum balticum, Glenodinium foliaceum, and Gyrodinium instriatum, after inoculation of that bacterial strain, cultures were obtained whose cell extracts showed the same kind of toxicity as G. tamarensis. No toxic action could be found in the extracts of the bacterial cells form the assayed strains. The interference of intracellular bacteria in the metabolism of dinoflagellates must be the main cause of their toxicity.
已记录到同一种甲藻藻华有的有毒，有的无毒，在后一种情况下，毒性可能有不同类型。已提出一种假说，即那些以某种方式参与毒素形成的细菌要么在甲藻细胞内，要么在培养液中。对这些微生物细胞内细菌的研究主要是在培养物材料中进行的，少数是在海洋样本中进行的，并且从不同物种中分离出了几个菌株。交叉接种实验表明，来自塔玛亚历山大藻的细菌菌株会使其他一些物种的细胞产生毒性。在用该细菌菌株接种后，从波罗原甲藻、叶状陀螺藻和条纹梨甲藻的无毒克隆培养物中获得了细胞提取物显示出与塔玛亚历山大藻相同毒性的培养物。在所检测菌株的细菌细胞提取物中未发现有毒作用。细胞内细菌对甲藻代谢的干扰必定是其毒性的主要原因。
