Papa P C, Moura C E B, Artoni L P, Fátima L A, Campos D B, Marques J E B, Baruselli P S, Binelli M, Pfarrer C, Leiser R
Department of Surgery, Sector of Anatomy, University of São Paulo, São Paulo, Brazil.
Domest Anim Endocrinol. 2007 Nov;33(4):379-89. doi: 10.1016/j.domaniend.2006.07.003. Epub 2006 Sep 18.
Water buffaloes are easily adaptable animals, whose raising and economical exploitation have been growing in the last three decades all over the world. Hyperstimulation of ovarian function in this species is a common technique aiming to improve reproductive performance. Superovulatory treatment affects corpus luteum (CL) function, which is highly correlated to angiogenic process. The aim of this study was therefore to assess the temporal protein and mRNA expression of VEGF and its receptors in the CL of non-treated and superovulated buffaloes. For that purpose blood samples and CL from 36 healthy (30 untreated, groups 1-5, and 6 superovulated, group 6) non-pregnant buffaloes were collected and the samples were divided into 6 groups according to the age of CL. Plasma samples were submitted to RIA to measure progesterone concentration and CL were subjected to immunohistochemistry and real time PCR for VEGF (vascular endothelial growth factor), Flt-1 (fms-like tyrosine kinase receptor 1) and KDR (kinase insert domain containing region). The VEGF system protein and mRNA expression during CL life span of untreated animals showed a specific time-dependent profile, although protein did not always reflect mRNA concentrations. VEGF expression in luteal cells was high correlated to plasma progesterone levels. Superovulated CL showed a significant increase of the VEGF-system protein and a significant decrease of mRNA expression compared to untreated animals in the same stage of the oestrous cycle. We conclude that VEGF, Flt-1 and KDR protein and mRNA expression in buffalo CL is dependent of estrous cycle stage and superovulatory treatment is able to increase the translation rate of this system.
水牛是适应性很强的动物，在过去三十年里，其养殖和经济开发在全球范围内不断增长。对该物种的卵巢功能进行超刺激是一种旨在提高繁殖性能的常用技术。超排处理会影响黄体（CL）功能，而黄体功能与血管生成过程高度相关。因此，本研究的目的是评估未处理和经超排处理的水牛黄体中血管内皮生长因子（VEGF）及其受体的蛋白质和mRNA表达随时间的变化情况。为此，采集了36头健康（30头未处理，分为1 - 5组，6头经超排处理，为第6组）未怀孕水牛的血液样本和黄体，并根据黄体的年龄将样本分为6组。血浆样本通过放射免疫分析（RIA）测定孕酮浓度，黄体则进行VEGF（血管内皮生长因子）、Flt - 1（fms样酪氨酸激酶受体1）和KDR（含激酶插入结构域区域）的免疫组织化学和实时定量PCR检测。未处理动物黄体寿命期间的VEGF系统蛋白质和mRNA表达呈现出特定的时间依赖性特征，尽管蛋白质表达并不总是反映mRNA浓度。黄体细胞中的VEGF表达与血浆孕酮水平高度相关。与处于相同发情周期阶段的未处理动物相比，经超排处理的黄体中VEGF系统蛋白质显著增加，而mRNA表达显著降低。我们得出结论，水牛黄体中VEGF、Flt - 1和KDR的蛋白质及mRNA表达依赖于发情周期阶段，超排处理能够提高该系统的翻译速率。
