Sharma H S, Nyberg F, Olsson Y, Dey P K
Laboratory of Neuropathology, University Hospital, Uppsala, Sweden.
Neuroscience. 1990;38(1):205-12. doi: 10.1016/0306-4522(90)90386-i.
The distribution of substance P was determined in the rat spinal cord and brain after a focal traumatic injury to the thoracic region (T10-11) of the spinal cord. There was at 1 and 2 h after the injury a statistically significant increase of the substance P content not only in the injured segment but also in samples removed 5 mm proximal (T9) and distal (T12) to the lesion. At 5 h the substance P content of the injured segment of the cord was reduced by 30% compared with controls. However, there was a significant increase in the concentration of this peptide in segments located 5 mm cranial and caudal to the injury (65% and 22%, respectively). Interestingly, the whole brain content of substance P showed a statistically significant 22% increase from control values at 5 h after the injury. At 1 and 2 h after the spinal cord injury there was a significant decrease in whole brain substance P concentration by 25% and 65%, respectively. Pretreatment with p-chlorophenylalanine (a serotonin synthesis inhibitor) markedly reduced the endogenous content of substance P in whole brain of normal animals. In these animals, the spinal cord content of the peptide was elevated by 83-123% as compared to untreated control animals. Spinal cord trauma inflicted on p-chlorophenylalanine-treated animals did not affect the brain peptide level at all. However, a profound decrease was noted in all the spinal cord segments at 5 h as compared to the untreated traumatized group. The decrease in this peptide was more pronounced in the cranial and the injured segments as compared to the caudal one.(ABSTRACT TRUNCATED AT 250 WORDS)
在大鼠脊髓胸段（T10 - 11）受到局灶性创伤后，测定了脊髓和脑中P物质的分布。损伤后1小时和2小时，不仅损伤节段的P物质含量有统计学意义的增加，而且在损伤部位近端5毫米（T9）和远端5毫米（T12）处采集的样本中P物质含量也增加。损伤后5小时，脊髓损伤节段的P物质含量与对照组相比降低了30%。然而，在损伤部位头侧和尾侧5毫米处的节段中，该肽的浓度有显著增加（分别增加65%和22%）。有趣的是，损伤后5小时，全脑P物质含量与对照值相比有统计学意义的22%的增加。脊髓损伤后1小时和2小时，全脑P物质浓度分别显著降低25%和65%。用对氯苯丙氨酸（一种血清素合成抑制剂）预处理可显著降低正常动物全脑中P物质的内源性含量。在这些动物中，与未处理的对照动物相比，该肽在脊髓中的含量升高了83% - 123%。对对氯苯丙氨酸处理的动物造成脊髓创伤根本不影响脑肽水平。然而，与未处理的创伤组相比，在5小时时所有脊髓节段均出现明显下降。与尾侧节段相比，该肽在头侧和损伤节段的下降更为明显。（摘要截短于250字）
