多克隆抗体和两种单克隆抗体与从卵巢浆液性肿瘤囊液中分离出的细胞亚群的反应性。
Reactivity of polyclonal and two monoclonal antibodies with cell subsets isolated from cystic fluids of ovarian serous neoplasms.
作者信息
Harłozińska A, Bar J K, Rabczyński J, Bast R C, Richter R, Cisło M
机构信息
Department of Tumor Immunology, Medical University Wrocław, Poland.
出版信息
Tumori. 1990 Oct 31;76(5):505-10. doi: 10.1177/030089169007600519.
The distribution of antigenic determinants on a cellular level in serous ovarian neoplasms was evaluated using polyclonal and two monoclonal antibodies (OC 125 and 10B). The expression of antigens was estimated by an immunofluorescence test on each cell fraction isolated by density centrifugation from cystic fluids of individual malignant and benign ovarian tumors, taking into account the density and cytomorphologic features of cell subpopulations. It was found that the studied antibodies recognized different antigenic determinants. Significant immunologic heterogeneity of cells among and within individual tumors was shown. Our studies show the value of isolated cell subpopulations for comparing the reactivity of different antibodies and estimating their immunodiagnostic potency.
利用多克隆抗体和两种单克隆抗体（OC 125和10B）评估了浆液性卵巢肿瘤细胞水平上抗原决定簇的分布。通过免疫荧光试验对从个体恶性和良性卵巢肿瘤囊液中经密度离心分离出的每个细胞组分进行抗原表达评估，同时考虑细胞亚群的密度和细胞形态学特征。结果发现，所研究的抗体识别不同的抗原决定簇。显示出个体肿瘤之间和内部细胞存在显著的免疫异质性。我们的研究表明，分离的细胞亚群对于比较不同抗体的反应性和评估其免疫诊断效力具有价值。
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